![Western Blot: Perforin Antibody (BOR21) [NBP1-43106] - Immunoblotting of human PBMC (Lane A) or PBMC stimulated with IL-2 for 3 days (Lane B) lysate; probed with 2 ug/mL Anti-Human Perforin Purified and anti-actin and revealed with Anti-Rat IgG HRP or Anti-Mouse IgG, respectively. Peforin is detected at ~72 k.](http://images.novusbio.com/fullsize/Perforin-Antibody-BOR21-Western-Blot-NBP1-43106-img0002.jpg "Western Blot: Perforin Antibody (BOR21) [NBP1-43106] - Immunoblotting of human PBMC (Lane A) or PBMC stimulated with IL-2 for 3 days (Lane B) lysate; probed with 2 ug/mL Anti-Human Perforin Purified and anti-actin and revealed with Anti-Rat IgG HRP or Anti-Mouse IgG, respectively. Peforin is detected at ~72 k.")
| Reactivity | HuSpecies Glossary |
| Applications | WB |
| Clone | BOR21 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 0.5 mg/ml |
| Immunogen | The immunogen for this antibody was Perforin. |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PRF1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | The BOR21 antibody has been tested for use in immunoblotting (WB) of human PBMCs at 1-5ug/ml. It is recommended that this antibody be titrated for optimal performance in the assay of interest. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS (pH 7.2) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Perforin Antibody (NBP1-43106)Find related products by research area.
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Perforin a Protective Serial Killer Secretory granule-mediated cell death is one of the the key mechanisms for elimination of virus-infected and transformed target cells by cytotoxic lymphocytes. Formation of the immunological synapse between an effector and a target cell leads to exocy... Read full blog post. |
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Perforin Antibodies Reveal Links to Apoptosis and Immune Response Perforin, also known as the pore-forming protein, pfp, is a 70 kD cytolytic protein expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. It is one of the major effector molecules used by CTL and NK cel... Read full blog post. |
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Perforin Antibodies for Detecting Immune System Diseases Perforin is a calcium-dependent pore forming cytolytic protein. Perforin is partially homologous to the terminal components of the membrane attack complex of complement and produces pores of up to 20nm in diameter on target membranes. Killer lymphocy... Read full blog post. |
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Perforin Antibodies Reveal Links to Apoptosis and Immune Response Perforin, also known as the pore-forming protein, pfp, is a 70 kD cytolytic protein expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. It is one of the major effector molecules used by CTL and NK cel... Read full blog post. |
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| Gene Symbol | PRF1 |
