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PDGFR alpha Antibody (APA5) [Biotin]

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Flow Cytometry: PDGF R alpha Antibody (APA5) [Biotin] [NBP1-43581] - Staining of NIH/3T3 cell line with 0.5 ug of Anti-Mouse CD140a (PDGF Receptor a) Biotin followed by Streptavidin PE . Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
Flow Cytometry: PDGF R alpha Antibody (APA5) [Biotin] [NBP1-43581] - Staining of NIH/3T3 cell line with 0.5 ug of Anti-Mouse CD140a (PDGF Receptor a) Biotin followed by Streptavidin PE . Appropriate isotype controls ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow
Clone
APA5
Clonality
Monoclonal
Host
Rat
Conjugate
Biotin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-43350AF350
Alexa Fluor 405
NBP1-43350AF405
Alexa Fluor 488
NBP1-43350AF488
Alexa Fluor 532
NBP1-43350AF532
Alexa Fluor 594
NBP1-43350AF594
Alexa Fluor 647
NBP1-43350AF647
Alexa Fluor 700
NBP1-43350AF700
Alexa Fluor 750
NBP1-43350AF750
Allophycocyanin
NBP1-43350APC
Allophycocyanin/Cy7
NBP1-43350APCCY7
Biotin
NBP1-43581-0.025mg
CoraFluor 1
NBP1-43350CL1
DyLight 350
NBP1-43350UV
DyLight 405
NBP1-43350V
DyLight 488
NBP1-43350G
DyLight 550
NBP1-43350R
DyLight 594
NBP1-43350DL594
DyLight 650
NBP1-43350C
DyLight 680
NBP1-43350FR
DyLight 755
NBP1-43350IR
FITC
NBP1-43350F
HRP
NBP1-43350H
Janelia Fluor 525
NBP1-43350JF525
Janelia Fluor 549
NBP1-43350JF549
Janelia Fluor 646
NBP1-43350JF646
Janelia Fluor 669
NBP1-43350JF669
PE
NBP1-43350PE
PE/Atto594
NBP1-43350PEATT594
PE/Cy5.5
NBP1-43350PECY55
PE/Cy7
NBP1-43350PECY7
PerCP
NBP1-43350PCP
Unconjugated
NBP1-43350-0.1mg
mFluor Violet 450 SE
NBP1-43350MFV450
mFluor Violet 500 SE
NBP1-43350MFV500
mFluor Violet 610 SE
NBP1-43350MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-43350-0.1mg
BSA Free
NBP1-43350-0.1mg
BSA Free
NBP1-43350-0.05mg
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PDGFR alpha Antibody (APA5) [Biotin] Summary

Immunogen
The immunogen for this antibody was CD140a.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
PDGFRA
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
The APA5 antibody has been tested by flow cytometric analysis of the NIH-3T3 cell line. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

In flow cytometry, we recommend using a cell surface staining protocol and fixation and permeabilization is not required to get staining. If fixation/permeabilization is needed for other markers in the same experiment, we recommend doing all surface staining prior to the fix/perm procedure. This antibody may not bind to a fixed epitope.
Readout System

Reactivity Notes

Human reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS (pH 7.2) with 0.1% gelatin
Preservative
0.09% Sodium Azide
Purity
Protein A or G purified

Alternate Names for PDGFR alpha Antibody (APA5) [Biotin]

  • alpha-type platelet-derived growth factor receptor
  • CD140 antigen-like family member A
  • CD140a antigen
  • CD140a
  • EC 2.7.10
  • EC 2.7.10.1
  • MGC74795
  • PDGF R alpha
  • PDGFR alpha
  • PDGFR2
  • PDGFRA
  • PDGFRA/BCR fusion
  • PDGF-R-alpha
  • platelet-derived growth factor receptor, alpha polypeptide
  • rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein
  • RHEPDGFRA

Background

The APA5 monoclonal antibody reacts with the mouse CD140a molecule, the alpha chain of the platelet derived growth factor receptor (PDGF receptor). PDGFRa is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of alpha/alpha, beta/beta, or heterodimers of alpha/beta and serve as a substrate for its kinase activity. CD140a is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PDGFRA