![Immunohistochemistry-Paraffin: PAX8 Antibody (ZR-1) - Azide and BSA Free [NBP3-08307] - Formalin-fixed, paraffin-embedded human kidney stained with PAX8 Recombinant Rabbit Monoclonal Antibody (ZR-1).](http://images.novusbio.com/fullsize/PAX8-Antibody-ZR-1-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08307-img0001.jpg "Immunohistochemistry-Paraffin: PAX8 Antibody (ZR-1) - Azide and BSA Free [NBP3-08307] - Formalin-fixed, paraffin-embedded human kidney stained with PAX8 Recombinant Rabbit Monoclonal Antibody (ZR-1).")
| Reactivity | HuSpecies Glossary |
| Applications | IHC |
| Clone | ZR-1 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07361). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Synthetic peptide corresponding to the C-terminus of human PAX8 protein (Uniprot: Q06710) |
| Localization | Nuclear |
| Marker | Renal Cell Marker |
| Specificity | Recognizes a protein of 62kDa, identified as PAX8. It is a member of the paired box (PAX) family of transcription factors. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas, and atypical thyroid adenomas. PAX-8 is expressed in the thyroid (and associated carcinomas), non-ciliated mucosal cells of the fallopian tubes, and simple ovarian inclusion cysts, but not normal ovarian surface epithelial cells. PAX-8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas. PAX-8 expression is reported in renal tubules as well as renal cell carcinoma, nephroblastoma, and seminoma. PAX-8 antibody may be used as an additional immunohistochemical marker for renal epithelial tumors. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | PAX8 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 62 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for PAX8 Antibody (NBP3-08307)Find related products by research area.
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| Gene Symbol | PAX8 |
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