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Patched 1/PTCH Antibody (OTI5C7) [DyLight 488]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI5C7
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 488

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-73253AF350
Alexa Fluor 405
NBP2-73253AF405
Alexa Fluor 488
NBP2-73253AF488
Alexa Fluor 532
NBP2-73253AF532
Alexa Fluor 594
NBP2-73253AF594
Alexa Fluor 647
NBP2-73253AF647
Alexa Fluor 700
NBP2-73253AF700
Alexa Fluor 750
NBP2-73253AF750
Allophycocyanin
NBP2-73253APC
Biotin
NBP2-73253B
CoraFluor 1
NBP2-73253CL1
DyLight 350
NBP2-73253UV
DyLight 405
NBP2-73253V
DyLight 550
NBP2-73253R
DyLight 594
NBP2-73253DL594
DyLight 650
NBP2-73253C
DyLight 680
NBP2-73253FR
DyLight 755
NBP2-73253IR
FITC
NBP2-73253F
HRP
NBP2-73253H
Janelia Fluor 525
NBP2-73253JF525
Janelia Fluor 549
NBP2-73253JF549
Janelia Fluor 646
NBP2-73253JF646
Janelia Fluor 669
NBP2-73253JF669
PE
NBP2-73253PE
PerCP
NBP2-73253PCP
Unconjugated
NBP1-47945
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-47945
Azide and BSA Free
NBP2-73253
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Patched 1/PTCH Antibody (OTI5C7) [DyLight 488] Summary

Immunogen
Recombinant protein fragment corresponding to amino acids 122-436 of human Patched 1/PTCH (NP_000255) produced in E.coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PTCH1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Patched 1/PTCH Antibody (OTI5C7) [DyLight 488]

  • BCNS
  • BCNSFLJ42602
  • FLJ26746
  • HPE7
  • mes
  • NBCCS
  • patched (Drosophila) homolog
  • Patched 1
  • patched homolog (Drosophila)
  • patched homolog 1 (Drosophila)
  • patched homolog 1
  • protein patched homolog 1
  • PTC
  • ptc1
  • PTCH protein +12b
  • PTCH protein +4'
  • PTCH protein -10
  • PTCH
  • PTCH1
  • PTCH11

Background

Mutations of the human Patched tumor suppressor gene (PTCH) have been identified in individuals with the nevoid basal cell carcinoma syndrome (NBCCS) as well as in sporadic basal cell carcinomas and medulloblastomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Patched 1/PTCH
Antibody (OTI5C7) [Dy ...
NBP2-73253G
Patched 1/PTCH Antibody (OTI5C7) [DyLight 488]
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Patched 1/PTCH Antibody (NBP2-73253G). (Showing 1 - 1 of 1 FAQ).

  1. I need a proven anti-human Ptch1 antibody that maps C-terminus of Ptch1 protein which is thus appropriate for all isoforms of human Ptch1 protein. Can you please suggest any which can be used for Western blotting?
    • NB100-41101 is expected to recognize all isoforms. NBP2-19705, NBP1-71662, 21130002, and NBP1-59455 should all suit your needs as well. Further details about each product can be found on their individual datasheets.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol PTCH1