![Western Blot: PAPOLA Antibody [NBP3-18653] - Figure 2. Western blot analysis using the antibody directed against PAPOLA Nuclear extracts from HeLa cells (40 ug) were analysed by Western blot using the antibody against PAPOLA diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 83 kDa); the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/PAPOLA-Antibody-Western-Blot-NBP3-18653-img0001.jpg "Western Blot: PAPOLA Antibody [NBP3-18653] - Figure 2. Western blot analysis using the antibody directed against PAPOLA Nuclear extracts from HeLa cells (40 ug) were analysed by Western blot using the antibody against PAPOLA diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 83 kDa); the marker (in kDa) is shown on the left.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Polyclonal antibody raised in rabbit against human PAPOLA (polyA polymerase alpha), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PAPOLA |
| Purity | Whole Antiserum |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
| Buffer | Whole antiserum |
| Preservative | 0.05% Sodium Azide |
| Purity | Whole Antiserum |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![ELISA: PAPOLA Antibody [NBP3-18653] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against human PAPOLA . The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:22,500.](http://images.novusbio.com/fullsize/PAPOLA Antibody-ELISA-NBP3-18653-img0002.jpg "ELISA: PAPOLA Antibody [NBP3-18653] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against human PAPOLA . The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:22,500.")
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