Home » p21/CIP1/CDKN1A » p21/CIP1/CDKN1A Antibodies » p21/CIP1/CDKN1A Antibody (195720) [Unconjugated]
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p21/CIP1/CDKN1A Antibody (195720) [Unconjugated]

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Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 16 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human p21/CIP1/CDKN1A Monoclonal Antibody (Catalog # MAB1047), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for p21/CIP1/CDKN1A at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 16 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human p21/CIP1/CDKN1A ...read more
p21/CIP1/CDKN1A was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human p21/ CIP1/CDKN1A Monoclonal Antibody (Catalog # MAB1047) at 15 µg/mL overnight at 4 °C. ...read more
MCF-7 human breast cancer cell line either treated with 1 µM camptothecin (CPT) for 16 hours (filled histogram) or untreated (open histogram) was stained with Mouse Anti-Human p21/CIP1/CDKN1A Monoclonal Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP, Flow
Clone
195720
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC1047U-100UG
Alexa Fluor 405
IC1047V-100UG
Alexa Fluor 488
IC1047G-100UG
Alexa Fluor 532
FAB1047X
Alexa Fluor 594
IC1047T-100UG
Alexa Fluor 647
IC1047R-100UG
Alexa Fluor 700
IC1047N-100UG
Alexa Fluor 750
IC1047S-100UG
Allophycocyanin
FAB1047A
Biotin
FAB1047B
DyLight 350
FAB1047D
DyLight 405
FAB1047E
DyLight 488
FAB1047K
DyLight 550
FAB1047L
DyLight 594
FAB1047M
DyLight 650
FAB1047W
DyLight 680
FAB1047Y
DyLight 755
FAB1047Z
FITC
FAB1047F
HRP
FAB1047H
Janelia Fluor 525
FAB1047JF525
Janelia Fluor 549
FAB1047I
Janelia Fluor 646
FAB1047J
Janelia Fluor 669
FAB1047JF669
PE
FAB1047P
PerCP
FAB1047C
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB1047-SP
Carrier Free
IC1047V-100UG
Carrier Free
IC1047U-100UG
Carrier Free
IC1047T-100UG
Carrier Free
IC1047S-100UG
Carrier Free
IC1047R-100UG
Carrier Free
IC1047N-100UG
Carrier Free
IC1047G-100UG
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p21/CIP1/CDKN1A Antibody (195720) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human p21/CIP1/CDKN1A
Ser2-Pro164
Accession # P38936
Specificity
Detects human p21/CIP1/CDKN1A in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CDKN1A
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation 4 ug/500 ug cell lysate
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 2 ug/mL
Publications
Read Publications using
MAB1047 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 μm filtered solution in PBS. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for p21/CIP1/CDKN1A Antibody (195720) [Unconjugated]

  • CAP20cyclin-dependent kinase inhibitor 1
  • CDK-interacting protein 1
  • CDKN1A
  • CDKN1melanoma differentiation associated protein 6
  • CIP1
  • CIP1WAF1CDK-interaction protein 1
  • cyclin-dependent kinase inhibitor 1A (p21, Cip1)
  • MDA6
  • MDA-6
  • Melanoma differentiation-associated protein 6
  • p21
  • p21CIP1
  • p21Cip1/Waf1
  • PIC1
  • SDI1DNA synthesis inhibitor
  • wild-type p53-activated fragment 1

Background

p21CIP1, also called CIP1 (CDK-interacting protein 1) and CDKN1A, is a 21 kDa Cyclin/Cyclin-dependent kinase (Cdk) inhibitor that blocks cell cycle progression from G1 to S phase in the cell cycle. Because p21 is a transcriptional target of the p53 tumor suppressor, p21 expression increases in cells that contain stabilized p53 due to genotoxic stress exposure.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for p21/CIP1/CDKN1A Antibody (MAB1047)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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(2)
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Bioinformatics

Gene Symbol CDKN1A
Uniprot