![Knockout Validated: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Wild-type (WT) and p21 Cip1 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody [GT1032]. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-1032-Western-Blot-NBP2-43697-img0005.jpg "Knockout Validated: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Wild-type (WT) and p21 Cip1 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody [GT1032]. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP, KO |
| Clone | 1032 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human p21/CIP1/CDKN1A. The exact sequence is proprietary. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CDKN1A |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 18 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for p21/CIP1/CDKN1A Antibody (NBP2-43697)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CDKN1A |
![Western Blot: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Analysis of: A. 30 ug HCT116 whole cell lysate/extract (untreated) B. 30 ug HCT116 whole cell lysate/extract (30 uM Cisplatin treatment for 24 hr) C. 30 ug HCT116 whole cell lysate/extract (30 uM Cisplatin treatment for 48 hr) 15 % SDS-PAGE p21 Cip1 antibody [1032].](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-1032-Western-Blot-NBP2-43697-img0003.jpg "Western Blot: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Analysis of: A. 30 ug HCT116 whole cell lysate/extract (untreated) B. 30 ug HCT116 whole cell lysate/extract (30 uM Cisplatin treatment for 24 hr) C. 30 ug HCT116 whole cell lysate/extract (30 uM Cisplatin treatment for 48 hr) 15 % SDS-PAGE p21 Cip1 antibody [1032].")
![Immunocytochemistry/Immunofluorescence: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Sample: Mock and treated HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p21 Cip1 stained by p21 Cip1 antibody [1032] diluted at 1:500.Blue: Hoechst 33342 staining. Scale bar= 10 um.](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-1032-Immunocytochemistry-Immunofluorescence-NBP2-43697-img0010.jpg "Immunocytochemistry/Immunofluorescence: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Sample: Mock and treated HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p21 Cip1 stained by p21 Cip1 antibody [1032] diluted at 1:500.Blue: Hoechst 33342 staining. Scale bar= 10 um.")
![Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Human lung cancer. p21 Cip1 stained by p21 Cip1 antibody [1032] diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-1032-Immunohistochemistry-Paraffin-NBP2-43697-img0007.jpg "Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Human lung cancer. p21 Cip1 stained by p21 Cip1 antibody [1032] diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Flow Cytometry: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - p21 Cip1 antibody [1032] detects p21 Cip1 by flow cytometry analysis. Sample: HepG2 cell. Black: Unlabelled sample was used as a control. Red: p21 Cip1 antibody [1032] dilution: 1:50. Acquisition of 20,000 events were collected for FACS analysis.](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-1032-Flow-Cytometry-NBP2-43697-img0009.jpg "Flow Cytometry: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - p21 Cip1 antibody [1032] detects p21 Cip1 by flow cytometry analysis. Sample: HepG2 cell. Black: Unlabelled sample was used as a control. Red: p21 Cip1 antibody [1032] dilution: 1:50. Acquisition of 20,000 events were collected for FACS analysis.")
![Immunocytochemistry/Immunofluorescence: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Analysis of Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDK4 protein stained by CDK4 antibody diluted at 1:1000. Red: p21 Cip1 protein stained by p21 Cip1 antibody [1032] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-1032-Immunocytochemistry-Immunofluorescence-NBP2-43697-img0004.jpg "Immunocytochemistry/Immunofluorescence: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Analysis of Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDK4 protein stained by CDK4 antibody diluted at 1:1000. Red: p21 Cip1 protein stained by p21 Cip1 antibody [1032] diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunoprecipitation: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Analysis of HCT-116 whole cell extract treat with 30uM cisplatin for 48 hr A. 30 ug HCT-116 whole cell extract treat with 30uM cisplatin for 48 hr B. Control with 4 ug of preimmune Mouse IgG C. Immunoprecipitation of CDKN1A protein by 4 ug p21 Cip1 antibody [1032] 15 % SDS-PAGE The immunoprecipitated CDKN1A protein was detected by p21 Cip1 antibody [1032] diluted at 1:1000. [EasyBlot anti-mouse IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-1032-Immunoprecipitation-NBP2-43697-img0002.jpg "Immunoprecipitation: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Analysis of HCT-116 whole cell extract treat with 30uM cisplatin for 48 hr A. 30 ug HCT-116 whole cell extract treat with 30uM cisplatin for 48 hr B. Control with 4 ug of preimmune Mouse IgG C. Immunoprecipitation of CDKN1A protein by 4 ug p21 Cip1 antibody [1032] 15 % SDS-PAGE The immunoprecipitated CDKN1A protein was detected by p21 Cip1 antibody [1032] diluted at 1:1000. [EasyBlot anti-mouse IgG was used as a secondary reagent]")
![Western Blot: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Wild-type (WT) and p21 Cip1 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody [GT1032] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-43697_mouse-monoclonal-p21-cip1-cdkn1a-antibody-1032-1610202419405732.jpg "Western Blot: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Wild-type (WT) and p21 Cip1 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody [GT1032] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
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