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p19ARF/CDKN2A Antibody [mFluor Violet 450 SE]

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Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
mFluor Violet 450 SE

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p19ARF/CDKN2A Antibody [mFluor Violet 450 SE] Summary

Immunogen
Synthetic peptide conjugated to KLH, derived from a sequence within residues 50-150 of mouse p19ARF/CDKN2A. [Swiss-Prot #Q64364]
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
Cdkn2a
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 31827530).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for p19ARF/CDKN2A Antibody [mFluor Violet 450 SE]

  • alternative reading frame
  • ARF
  • ARF-INK4a
  • CDK4I
  • CDKN2A
  • Cyclin-dependent kinase 4 inhibitor A
  • cyclin-dependent kinase inhibitor 2A (p16, inhibits CDK4)
  • cyclin-dependent kinase inhibitor 2A
  • cyclin-dependent kinase inhibitor 2A, isoform 3
  • cyclin-dependent kinase inhibitor 2A, isoforms 1/2
  • cyclin-dependent kinase inhibitor protein
  • INK4
  • INK4a
  • Ink4a/Arf
  • INK4a-ARF
  • mitochondrial smARF'
  • MTS1
  • p14
  • p14ARF
  • P16
  • p16(INK4a)
  • p16-INK4
  • P16INK4A
  • p16-INK4a
  • P19
  • p19<ARF>
  • p19ARF
  • Pctr1

Background

p19ARF (mouse) and p14ARF (human) has become an important player in cell cycle regulation. In mice, tumor suppressor effects appear to be mediated by interactions between p19ARF and the p53 tumor suppressor protein. p19ARF counters uncontrolled proliferation and oncogenic signals in p53 dependent pathways. Proteins encoded by the INK4a/ARF locus also play a role in Abelson virus transformation. Both p16INK4a and p19ARF are expressed in many cells as they emerge from the apoptotic crisis that characterizes the transformation process. p19ARF is an important part in cellular defense against transforming signals from the Abl oncoprotein, providing direct evidence that the p19ARF-p53 regulatory loop plays an important role in lymphoma induction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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mFluor Violet Conjugated Antibodies

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Bioinformatics

Gene Symbol Cdkn2a