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p19ARF/CDKN2A Antibody [Janelia Fluor® 525]

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Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Janelia Fluor 525

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB200-106AF350
Alexa Fluor 405
NB200-106AF405
Alexa Fluor 488
NB200-106AF488
Alexa Fluor 532
NB200-106AF532
Alexa Fluor 594
NB200-106AF594
Alexa Fluor 647
NB200-106AF647
Alexa Fluor 700
NB200-106AF700
Alexa Fluor 750
NB200-106AF750
Allophycocyanin
NB200-106APC
Biotin
NB200-106B
CoraFluor 1
NB200-106CL1
DyLight 350
NB200-106UV
DyLight 405
NB200-106V
DyLight 488
NB200-106G
DyLight 550
NB200-106R
DyLight 594
NB200-106DL594
DyLight 650
NB200-106C
DyLight 680
NB200-106FR
DyLight 755
NB200-106IR
FITC
NB200-106F
HRP
NB200-106H
Janelia Fluor 549
NB200-106JF549
Janelia Fluor 585
NB200-106JF585
Janelia Fluor 635
NB200-106JF635
Janelia Fluor 646
NB200-106JF646
Janelia Fluor 669
NB200-106JF669
PE
NB200-106PE
PerCP
NB200-106PCP
Unconjugated
NB200-106
mFluor Violet 450 SE
NB200-106MFV450
mFluor Violet 500 SE
NB200-106MFV500
mFluor Violet 610 SE
NB200-106MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB200-106
BSA Free
NB200-106SS
BSA Free
NB200-106
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p19ARF/CDKN2A Antibody [Janelia Fluor® 525] Summary

Immunogen
Synthetic peptide conjugated to KLH, derived from a sequence within residues 50-150 of mouse p19ARF/CDKN2A. [Swiss-Prot #Q64364]
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
Cdkn2a
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 31827530).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for p19ARF/CDKN2A Antibody [Janelia Fluor® 525]

  • alternative reading frame
  • ARF
  • ARF-INK4a
  • CDK4I
  • CDKN2A
  • Cyclin-dependent kinase 4 inhibitor A
  • cyclin-dependent kinase inhibitor 2A (p16, inhibits CDK4)
  • cyclin-dependent kinase inhibitor 2A
  • cyclin-dependent kinase inhibitor 2A, isoform 3
  • cyclin-dependent kinase inhibitor 2A, isoforms 1/2
  • cyclin-dependent kinase inhibitor protein
  • INK4
  • INK4a
  • Ink4a/Arf
  • INK4a-ARF
  • mitochondrial smARF'
  • MTS1
  • p14
  • p14ARF
  • P16
  • p16(INK4a)
  • p16-INK4
  • P16INK4A
  • p16-INK4a
  • P19
  • p19<ARF>
  • p19ARF
  • Pctr1

Background

p19ARF (mouse) and p14ARF (human) has become an important player in cell cycle regulation. In mice, tumor suppressor effects appear to be mediated by interactions between p19ARF and the p53 tumor suppressor protein. p19ARF counters uncontrolled proliferation and oncogenic signals in p53 dependent pathways. Proteins encoded by the INK4a/ARF locus also play a role in Abelson virus transformation. Both p16INK4a and p19ARF are expressed in many cells as they emerge from the apoptotic crisis that characterizes the transformation process. p19ARF is an important part in cellular defense against transforming signals from the Abl oncoprotein, providing direct evidence that the p19ARF-p53 regulatory loop plays an important role in lymphoma induction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol Cdkn2a