p19ARF/CDKN2A Antibody (5-C3-1) [PE]

ARF (alternative reading frame; p14ARF in human, p19ARF in mouse) is a tumor suppressor that serves as a sensor of hyper-proliferative signals, resulting in p53-dependent growth arrest and apoptotic cell death. ARF interacts with several proteins including COMMD1, CDKN2AIP, TBRG1, E4F1, C1QBP etc. and can bind to BCL6, E2F1, HUWE1, MDM2, MYC, NPM1/B23, TOP1/TOPOI as well as UBE2I/UBC9. ARF has the capability of inducing cell cycle arrest in G1 as well as G2 phases. While ARF is not expressed at significant levels in most normal tissues, oncogene activation triggers ARF overexpression, resulting in inhibition of MDM2 ubiquitin ligase and stabilization of p53. It is induced by progesterone as well as Ras/DMTF1, and is downregulated through NFkB p65 subunit (RELA). Inhibition of the p53 pathway, most commonly via mutations in p53 itself, inactivation of ARF, or amplification of MDM2 has been proposed to be a crucial step in pathogenesis of most human malignancies. Moreover, mice with null ARF have been shown to develop tumors rapidly and to display delayed mammary gland involution.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Showing Publication 1 - 1 of 1.

Publication using NB200-174PE	Applications	Species
Santelli A, Sun I, Eirin A, Abumoawad A, Woollard J, Lerman A, Textor S, Puranik A, Lerman L Senescent Kidney Cells in Hypertensive Patients Release Urinary Extracellular Vesicles. J Am Heart Assoc, 2019-06-01;8(11):e012584. 2019-06-01 [PMID: 31433703]

Images	Ratings	Applications	Species	Date	Details
	5 reviewed by: Anja Kusch	Flow	Mouse	11/28/2024	View Summary Application Flow Cytometry Sample Tested Thymus tissue Species Mouse Lot D177076 Comments Comments Conducted the Antibody trial with two different dilutions (1:500 and 1:100) and compared them to an IgG control which was also PE labelled. The staining worked very nicely in all cases upon fixation with the Foxp3 staining kit.

Array NB200-174PE

• p19ARF/CDKN2A Antibodies
• p19ARF/CDKN2A ELISA Kits
• p19ARF/CDKN2A Protein