Home » p19ARF/CDKN2A » p19ARF/CDKN2A Antibodies » p19ARF/CDKN2A Antibody (5-C3-1) [Janelia Fluor® 549]
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p19ARF/CDKN2A Antibody (5-C3-1) [Janelia Fluor® 549]

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Flow Cytometry: p19ARF/CDKN2A Antibody (5-C3-1) [Janelia Fluor® 549] - Conducted the antibody trial with two different dilutions of p19ARF/CDKN2A Antibody (5-C3-1) [PE] (Catalog # NB200-174PE) (1:500 and 1:100) and compared them to an IgG control which was also PE labelled. Image from a verified customer review.
Flow Cytometry: p19ARF/CDKN2A Antibody (5-C3-1) [Janelia Fluor® 549] - Conducted the antibody trial with two different dilutions of p19ARF/CDKN2A Antibody (5-C3-1) [PE] (Catalog # NB200-174PE) (1:500 and 1:100) and ...read more

Product Details

Summary
Reactivity Hu, Mu, SyHa(-)Species Glossary
Applications WB, Flow, ICC/IF, IHC, IP, CyTOF-ready, KO
Clone
5-C3-1
Clonality
Monoclonal
Host
Rat
Conjugate
Janelia Fluor 549

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB200-174AF350
Alexa Fluor 405
NB200-174AF405
Alexa Fluor 488
NB200-174AF488
Alexa Fluor 532
NB200-174AF532
Alexa Fluor 594
NB200-174AF594
Alexa Fluor 647
NB200-174AF647
Alexa Fluor 700
NB200-174AF700
Alexa Fluor 750
NB200-174AF750
Allophycocyanin
NB200-174APC
Allophycocyanin/Cy7
NB200-174APCCY7
Biotin
NB200-174B
CoraFluor 1
NB200-174CL1
DyLight 350
NB200-174UV
DyLight 405
NB200-174V
DyLight 488
NB200-174G
DyLight 550
NB200-174R
DyLight 594
NB200-174DL594
DyLight 650
NB200-174C
DyLight 680
NB200-174FR
DyLight 755
NB200-174IR
FITC
NB200-174F
HRP
NB200-174H
Janelia Fluor 525
NB200-174JF525
Janelia Fluor 646
NB200-174JF646
Janelia Fluor 669
NB200-174JF669
PE
NB200-174PE
PE/Atto594
NB200-174PEATT594
PE/Cy5.5
NB200-174PECY55
PE/Cy7
NB200-174PECY7
PerCP
NB200-174PCP
Unconjugated
NB200-174
mFluor Violet 450 SE
NB200-174MFV450
mFluor Violet 500 SE
NB200-174MFV500
mFluor Violet 610 SE
NB200-174MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB200-174
BSA Free
NB200-174SS
BSA Free
NB200-174
Azide and BSA Free
NBP2-80897
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p19ARF/CDKN2A Antibody (5-C3-1) [Janelia Fluor® 549] Summary

Immunogen
A synthetic peptide containing amino acids 54-75 of the murine p19ARF/CDKN2A. [UniProt# Q64364]
Epitope
The epitope recognized is between amino acids 62-75 of murine p19ARF/CDKN2A.
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
Cdkn2a
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Knockout Validated
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 27177758). The antibody does not react with Golden Syrian hamster. Armenian hamster has not been tested.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for p19ARF/CDKN2A Antibody (5-C3-1) [Janelia Fluor® 549]

  • alternative reading frame
  • ARF
  • ARF-INK4a
  • CDK4I
  • CDKN2A
  • Cyclin-dependent kinase 4 inhibitor A
  • cyclin-dependent kinase inhibitor 2A (p16, inhibits CDK4)
  • cyclin-dependent kinase inhibitor 2A
  • cyclin-dependent kinase inhibitor 2A, isoform 3
  • cyclin-dependent kinase inhibitor 2A, isoforms 1/2
  • cyclin-dependent kinase inhibitor protein
  • INK4
  • INK4a
  • Ink4a/Arf
  • INK4a-ARF
  • mitochondrial smARF'
  • MTS1
  • p14
  • p14ARF
  • P16
  • p16(INK4a)
  • p16-INK4
  • P16INK4A
  • p16-INK4a
  • P19
  • p19<ARF>
  • p19ARF
  • Pctr1

Background

p19ARF (mouse) and p14ARF (human) has become an important player in cell cycle regulation. In mice, tumor suppressor effects appear to be mediated by interactions between p19ARF and the p53 tumor suppressor protein. p19ARF counters uncontrolled proliferation and oncogenic signals in p53 dependent pathways. Proteins encoded by the INK4a/ARF locus also play a role in Abelson virus transformation. Both p16INK4a and p19ARF are expressed in many cells as they emerge from the apoptotic crisis that characterizes the transformation process. p19ARF is an important part in cellular defense against transforming signals from the Abl oncoprotein, providing direct evidence that the p19ARF-p53 regulatory loop plays an important role in lymphoma induction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol Cdkn2a