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Human p16INK4a/CDKN2A ELISA Kit (Colorimetric)

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ELISA: Human p16INK4a/CDKN2A ELISA Kit (Colorimetric) [NBP2-75209] - Samples were spiked with high concentrations of Human p16INK4a/CDKN2A and diluted with Reference Standard & Sample Diluent to produce samples with ...read more
ELISA: Human p16INK4a/CDKN2A ELISA Kit (Colorimetric) [NBP2-75209] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.63 - 40 ng/mL
Sensitivity
0.38 ng/mL

Order Details

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Human p16INK4a/CDKN2A ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human CDKN2A (p16-INK4a, p16 isoform) in samples. No significant cross-reactivity or interference between Human CDKN2A and analogues was observed.
Standard Curve Range
0.63 - 40 ng/mL
Sensitivity
0.38 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.24%
Intra-Assay
CV% < 5.32%
Spike Recovery
86-107%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CDKN2A

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human p16INK4a/CDKN2A ELISA Kit (Colorimetric)

  • ARF
  • CDK4 inhibitor p16-INK4
  • CDK4I
  • CDK4IP14ARF
  • CDKN2
  • CDKN2A
  • cell cycle negative regulator beta
  • CMM2P16-INK4A
  • Cyclin-dependent kinase 4 inhibitor A
  • cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)
  • cyclin-dependent kinase inhibitor 2A
  • INK4
  • INK4a
  • MLM
  • MLMP16INK4
  • MTS-1
  • MTS1P14
  • Multiple tumor suppressor 1
  • p14
  • p14ARF
  • p16
  • p16-INK4
  • p16INK4a
  • p16-INK4a
  • P19
  • p19ARF
  • TP16

Background

CDKN2A/p16INK4a is a gene that codes for a widely expressed protein with four isoforms, measuring 156, 105, 116, and 167 amino acids in length with weights of approximately 17, 11, 12, and 18 kDa respectively. CDKN2A/p16INK4a functions as a negative regulator of the proliferation of cells, which then allows them to interact with cyclins D and to phosphorylate the retinoblastoma protein. Current studies are being done on several diseases and disorders related to this gene including Li-Fraumeni syndrome, malignant peripheral nerve sheath tumor, blastic plasmacytoid dendritic cell, acth-secreting pituitary adenoma, recessive dystrophic epidermolysis bullosa, marginal zone b-cell lymphoma, adult astrocytic tumor, melanoma, and squamous cell carcinoma. CDKN2A/p16INK4a has also been shown to have interactions with MDM2, ZNF420, EGR1, MYCN, and CDK6 in pathways such as the cell cycle, p53 signaling, mitochondrial apoptosis, HIF-1 Alpha, CRHR, pancreatic cancer, and glioma pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for p16INK4a/CDKN2A ELISA Kit (NBP2-75209). (Showing 1 - 1 of 1 FAQ).

  1. Our customer asked us about NBP1-02905, which we lately found it discontinued. While checking its related products, there seems none. Would you please help confirm if there is any replacement available?
    • With regards to your question on our CDKN2A antibodies, we do supply a wide range of products, but you haven't specified which application you are referring to. Please go through this link (cyclin-dependent kinase inhibitor 2A) and choose the product that will suit your experiment and the species you are working with. Meanwhile it is important to keep in mind that CDKN2A generates several transcript variants which differ in their first exons.

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Bioinformatics

Gene Symbol CDKN2A