p14ARF/CDKN2A Antibody (4C6/4) - Azide and BSA Free Summary
Description |
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07234).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
Immunogen |
Recombinant full-length human p14ARF/CDKN2A protein (Uniprot: P42771) |
Localization |
Nuclear and Cytoplasmic |
Isotype |
IgG2a Kappa |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
CDKN2A |
Purity |
Protein A or G purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Flow Cytometry 1-2 ug/million cells
- Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
- Immunohistochemistry
- Immunohistochemistry-Paraffin
- Western Blot 1-2 ug/ml
|
Application Notes |
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. |
Theoretical MW |
14 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at -20 to -80C. Avoid freeze-thaw cycles. |
Buffer |
10 mM PBS |
Preservative |
No Preservative |
Concentration |
1 mg/ml |
Purity |
Protein A or G purified |
Alternate Names for p14ARF/CDKN2A Antibody (4C6/4) - Azide and BSA Free
Background
P14ARF is an alternate reading frame (ARF) product of the CDKN2A locus, which acts as a tumor suppressor by inducing cell cycle arrest in G1 and G2 phases and apoptosis.
The INK4a-ARF locus is comprised of two tumor suppressors, p16INK4a and p14ARF. These two proteins are encoded by differential splicing of alternative first exons. The p16INK4a (exon 1 alpha) protein inhibits the cyclin D-dependent kinases (CDK) that control the phosphorylation of the Rb protein and cell proliferation. The p14ARF protein complexes with MDM2 within the nucleus, thus modulating the activity of the p53 protein. P14ARF is a potent tumor suppressor in the presence of wild-type p53, while mutant p53 substantially reduces growth inhibition by p14ARF.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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FAQs for p14ARF/CDKN2A Antibody (NBP3-08233) (0)
Secondary Antibodies
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