Orthogonal Strategies: Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038] - Staining in human liver and tonsil tissues. Corresponding OTC RNA-seq data are presented for ...read more
Western Blot: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038] - Analysis in human liver tissue.
Immunocytochemistry/ Immunofluorescence: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038] - Staining of RH-30 cells using the Anti-OTC monoclonal antibody, showing specific staining in mitochondria in ...read more
Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038] - Staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038] - Staining of human small intestine shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038] - Staining of human tonsil shows no positivity in lymphoid cells as expected.
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence:ILADYLTLQEHYSSLKGLTLSWIGDGNNILHSIMMSAAKFGMHLQAATPKGYEPDASVTKLAEQYAKENGTKLLLTNDPLEAAHGGNVLITDTWISMGQEEEKKKRLQAFQGYQVTMKTAKVAASDWT
Epitope
GYEPDASVTKLAEQY
Predicted Species
Mouse (93%), Rat (92%). Backed by our 100% Guarantee.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
OTC
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use in Cynomolgus Monkey reported in scientific literature (PMID:35211641). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified
Alternate Names for Ornithine Carbamoyltransferase Antibody (CL4045)
EC 2.1.3
EC 2.1.3.3
MGC129967
MGC129968
MGC138856
OCTD
Ornithine Carbamoyltransferase
ornithine carbamoyltransferase, mitochondrial
Ornithine Transcarbamylase
OTC
OTCase
Background
This nuclear gene encodes a mitochondrial matrix enzyme. Missense, nonsense, and frameshift mutations in this enzyme lead to ornithine transcarbamylase deficiency, which causes hyperammonemia. Since the gene for this enzyme maps close to that for Duchenne muscular dystrophy, it may play a role in that disease also. (provided by RefSeq)
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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