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Opsin 3 Antibody - BSA Free

Images

 
Immunohistochemistry Free-Floating: Opsin 3 Antibody [NLS2134] - Rat brain samples (cerebral cortex). Rat brain 40um free floating sections were incubated with Opsin 3 Antibody (NLS2134) at 1:250 overnight at 4 degrees ...read more
Immunohistochemistry-Paraffin: Opsin 3 Antibody [NLS2134] - Analysis of anti-Encephalopsin / OPN3 antibody with brain, neurons and glia at 5 ug/mL.
Immunohistochemistry-Paraffin: Opsin 3 Antibody [NLS2134] - Analysis of anti-Encephalopsin / OPN3 antibody with human skin, melanoma.
Immunohistochemistry-Paraffin: Opsin 3 Antibody [NLS2134] - Anti-OPN3 / Encephalopsin antibody IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, PmSpecies Glossary
Applications IHC, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

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Opsin 3 Antibody - BSA Free Summary

Description
Product can be stored undiluted at 4C for up to 1 month.
Immunogen
Synthetic 16 amino acid peptide from C-terminus of human Opsin 3.
Epitope
C-Terminus
Localization
Cell Membrane
Specificity
Human Opsin 3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Species
Monkey (94%), Primate (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
OPN3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 - 8 ug/mL
Application Notes
Use in Immunohistochemistry reported in scientific literature (PMID: 21700660). This Opsin 3 Antibody is validated for IHC-FrFl from a verified customer review.
Reviewed Applications
Read 1 Review rated 3
using
NLS2134 in the following applications:

Publications
Read Publication using
NLS2134 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 21700660). Predicted cross-reactivity based on sequence identity: Gibbon (94%), Marmoset (94%). Rat reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Buffer
PBS
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Opsin 3 Antibody - BSA Free

  • ECPN
  • ECPNopsin-3
  • encephalopsin
  • ERO
  • NMO-1
  • opsin 3 (encephalopsin, panopsin)
  • opsin 3
  • Panopsin

Background

Encephalopsin, a member of the Opsin subfamily of GPCRs, is similar in sequence to vertebrate retinal and pineal opsins and may affect photoreception in the brain. Encephalopsin expression has been documented in various brain tissues, including the preoptic area and paraventricular nucleus of the hypothalamus, cerebral cortex, cerebellar Purkinje neurons, a subset of striatal neurons, selected thalamic nuclei, and a subset of interneurons in the ventral horn of the spinal cord. ESTs have been isolated from human blood, breast, cervix, colon, germ cell, head/neck, kidney, lung, pancreas, placenta, prostate, skin, testis, and uterus cancer libraries, as well as from normal human blood, brain, eye, heart, kidney, liver, liver/spleen, ovary, placenta, prostate, skin, testis, and B-cell/lung/testis libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: IHC, IHC

Publications for Opsin 3 Antibody (NLS2134)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IF/IHC.


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IF/IHC
(1)
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Mouse
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Review for Opsin 3 Antibody (NLS2134) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NLS2134:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Rat
(1)
All Species
Images Ratings Applications Species Date Details
  3
reviewed by:
Soledad Barez
IHC-Fr Rat 10/26/2021
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedBrain free floating slices
SpeciesRat
Lot41006

Comments

CommentsRat brain 40um free floating sections were incubated with Opsin 3 Antibody (NLS2134) at 1:250 overnight at 4°C. The following day, sections were incubated with anti-rabbit Alexa Fluor Plus 488 1:500 and DAPI.
***Novus Innovators Program - new species or application used on a primary antibody.***

Product General Protocols

View specific protocols for Opsin 3 Antibody (NLS2134): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Opsin 3 Antibody (NLS2134) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

3
5
0
4
0
3
1
2
0
1
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Soledad Barez
10/26/2021
Application: IHC-Fr
Species: Rat

Bioinformatics

Gene Symbol OPN3
Entrez