![Western Blot: O-GlcNAc Transferase p110 subunit Antibody [NB100-57845] - Rat pancreas lysate (35 ug protein in RIPA buffer). Primary antibody at 0.05 ug/mL, 1 hr incubation. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/O-GlcNAc-Transferase-p110-subunit-Antibody-Western-Blot-NB100-57845-img0002.jpg "Western Blot: O-GlcNAc Transferase p110 subunit Antibody [NB100-57845] - Rat pancreas lysate (35 ug protein in RIPA buffer). Primary antibody at 0.05 ug/mL, 1 hr incubation. Detected by chemiluminescence.")
| Reactivity | Hu, Rt, Mu, Bv, CaSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-YEHPKDLKLSDGR, from the internal region of the protein sequence according to NP_858058.1; NP_858059.1. |
| Epitope | YEHPKDLKLSDGR |
| Specificity | This antibody is expected to recognise both reported isoforms (NP_858058.1 and NP_858059.1 |
| Predicted Species | Mouse (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | OGT |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB: Approx 110k Da band observed in pancreas lysates from rat (calculated MW of 117 kDa according to NP_858058.1. An additional band of unknown identity was also consistently observed at 60 kDa. This band was successfully blocked by incubation with the immunizing peptide. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NB100-57845 | Applications | Species |
|---|---|---|
| Majumdar G, Wright J, Markowitz P et al. Insulin stimulates and diabetes inhibits O-linked N-acetylglucosamine transferase and O-glycosylation of Sp1. Diabetes 2004-12-01 [PMID: 15561949] |
Secondary Antibodies |
Isotype Controls |
Research Areas for O-GlcNAc Transferase p110 subunit Antibody (NB100-57845)Find related products by research area.
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| Gene Symbol | OGT |
| Entrez | |
| Uniprot |
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![Immunocytochemistry/Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody [NB100-57845] - Paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and membrane/cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/O-GlcNAc-Transferase-p110-subunit-Antibody-Immunocytochemistry-Immunofluorescence-NB100-57845-img0005.jpg "Immunocytochemistry/Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody [NB100-57845] - Paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and membrane/cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NB100-57845] - Human cortex tissue. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/O-GlcNAc-Transferase-p110-subunit-Antibody-Immunohistochemistry-Paraffin-NB100-57845-img0001.jpg "Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NB100-57845] - Human cortex tissue. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Flow Cytometry: O-GlcNAc Transferase p110 subunit Antibody [NB100-57845] - Paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/O-GlcNAc-Transferase-p110-subunit-Antibody-Flow-Cytometry-NB100-57845-img0006.jpg "Flow Cytometry: O-GlcNAc Transferase p110 subunit Antibody [NB100-57845] - Paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.")
![Immunocytochemistry/Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody [NB100-57845] - Paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/O-GlcNAc-Transferase-p110-subunit-Antibody-Immunocytochemistry-Immunofluorescence-NB100-57845-img0003.jpg "Immunocytochemistry/Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody [NB100-57845] - Paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Immunocytochemistry/Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody [NB100-57845] - Paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/O-GlcNAc-Transferase-p110-subunit-Antibody-Immunocytochemistry-Immunofluorescence-NB100-57845-img0004.jpg "Immunocytochemistry/Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody [NB100-57845] - Paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
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