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FEZ1 Antibody

Images

 
Western Blot: FEZ1 Antibody [NB100-53816] - Staining (0.1ug/ml) of Kelly cell lysate (A) + Peptide (B) (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: FEZ1 Antibody [NB100-53816] - Negative Control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.
Western Blot: FEZ1 Antibody [NB100-53816] - Staining (0.1ug/ml) of Rat Brain lysate (A) + Peptide (B) (35g protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: FEZ1 Antibody [NB100-53816] - Staining (5µg/ml) of paraffin embedded Human Cerebellum. Heat induced antigen retrieval with citrate buffer pH 6, HRP-Staining.

Product Details

Summary
Reactivity Hu, Rt, Mu, CaSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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FEZ1 Antibody Summary

Immunogen
Peptide with sequence C-QNKQKEQRELMKK corresponding to internal region according to NP_005094.1.
Specificity
This antibody is expected to recognize isoform 1 (NP_005094.1) only.
Predicted Species
Mouse (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FEZ1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5 - 8 ug/ml
  • Immunohistochemistry-Paraffin 5 - 8 ug/ml
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.1 - 0.3 ug/ml
Application Notes
WB: Approx 75 kDa band observed in Kelly and in Rat Brain lysates (calculated MW of 45.1 kDa according to NP_005094.1 and 45. 2kDa according to NP_112328.1)
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
FEZ1 Overexpression Lysate
Human Brain Frontal Lobe Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publications using NB100-53816.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FEZ1 Antibody

  • fasciculation and elongation protein zeta 1 (zygin I)
  • fasciculation and elongation protein zeta-1
  • Zygin I
  • zygin-1

Background

This gene is an ortholog of the C. elegans unc-76 gene, which is necessary for normal axonal bundling and elongation within axon bundles. Expression of this gene in C. elegans unc-76 mutants can restore to the mutants partial locomotion and axonal fasciculation, suggesting that it also functions in axonal outgrowth. The N-terminal half of the gene product is highly acidic. Alternatively spliced transcript variants encoding different isoforms of this protein have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FEZ1 Antibody (NB100-53816)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FEZ1
Entrez
Uniprot