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NSUN2 Antibody

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Genetic Strategies: Western Blot: NSUN2 Antibody [NBP1-82189] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-NSUN2 antibody. Remaining relative ...read more
Immunohistochemistry-Paraffin: NSUN2 Antibody [NBP1-82189] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: NSUN2 Antibody [NBP1-82189] - Staining of human duodenum shows moderate nuclear and cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: NSUN2 Antibody [NBP1-82189] - Staining of human skeletal muscle shows very weak nuclear positivity in myocytes as expected.
Immunohistochemistry-Paraffin: NSUN2 Antibody [NBP1-82189] - Staining of human skin shows weak to moderate nuclear positivity in squamous epithelial cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

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NSUN2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PFVFIPEDDPLFPPIEKFYALDPSFPRMNLLTRTTEGKKRQLYMVSKELRNVLLNNSEKMKVINTGIKVWCRNNSGEEFDCAFRLAQ
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NSUN2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500-1:1000
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
NSUN2 Protein (NBP1-82189PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NSUN2 Antibody

  • EC 2.1.1
  • EC 2.1.1.29
  • FLJ20303,5-methycytoisine methyltransferase
  • hTrm4
  • member 2
  • Myc-induced SUN-domain-containing protein
  • NOL1/NOP2/Sun domain family 2
  • NOL1/NOP2/Sun domain family member 2
  • NOP2/Sun domain family, member 2
  • SAKIMisu
  • Substrate of AIM1/Aurora kinase B
  • TRM4MISU
  • tRNA (cytosine-5-)-methyltransferase NSUN2
  • tRNA (cytosine-5-)-methyltransferase
  • tRNA methyltransferase 4 homolog

Background

Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu(CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 encodes a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu(CAA) (Brzezicha et al., 2006 [PubMed 17071714]).[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol NSUN2