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NRAGE/MAGED1 Antibody (353124) [Unconjugated]

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NRAGE was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Human NRAGE Monoclonal Antibody (Catalog # MAB3835) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
NRAGE was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Human NRAGE Monoclonal Antibody (Catalog # MAB3835) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready, ICC/IF
Clone
353124
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB3835U-100UG
Alexa Fluor 405
FAB3835V-100UG
Alexa Fluor 488
FAB3835G-100UG
Alexa Fluor 532
FAB3835X
Alexa Fluor 594
FAB3835T-100UG
Alexa Fluor 647
FAB3835R-100UG
Alexa Fluor 700
FAB3835N-100UG
Alexa Fluor 750
FAB3835S-100UG
Allophycocyanin
FAB3835A
Allophycocyanin/Cy7
FAB3835APCCY7
Biotin
FAB3835B
CoraFluor 1
FAB3835CL1
DyLight 350
FAB3835D
DyLight 405
FAB3835E
DyLight 488
FAB3835K
DyLight 550
FAB3835L
DyLight 594
FAB3835M
DyLight 650
FAB3835W
DyLight 680
FAB3835Y
DyLight 755
FAB3835Z
FITC
FAB3835F
HRP
FAB3835H
Janelia Fluor 525
FAB3835JF525
Janelia Fluor 549
FAB3835I
Janelia Fluor 585
FAB3835JF585
Janelia Fluor 635
FAB3835JF635
Janelia Fluor 646
FAB3835J
Janelia Fluor 669
FAB3835JF669
PE
FAB3835P
PE/Atto594
FAB3835PEATT594
PE/Cy5.5
FAB3835PECY55
PE/Cy7
FAB3835PECY7
PerCP
FAB3835C
Unconjugated
FAB3835A
mFluor Violet 450 SE
FAB3835MFV450
mFluor Violet 500 SE
FAB3835MFV500
mFluor Violet 610 SE
FAB3835MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
FAB3835A
Carrier Free
MAB3835-SP
Carrier Free
FAB3835V-100UG
Carrier Free
FAB3835U-100UG
Carrier Free
FAB3835T-100UG
Carrier Free
FAB3835S-100UG
Carrier Free
FAB3835R-100UG
Carrier Free
FAB3835N-100UG
Carrier Free
FAB3835G-100UG
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NRAGE/MAGED1 Antibody (353124) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human NRAGE
Met1-Glu778
Accession # Q9Y5V3
Specificity
Detects human NRAGE in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
MAGED1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NRAGE/MAGED1 Antibody (353124) [Unconjugated]

  • Dlxin-1
  • MAGE tumor antigen CCF
  • MAGED1
  • melanoma antigen family D, 1
  • melanoma-associated antigen D1
  • Neurotrophin receptor-interacting MAGE homolog
  • NRAGE MAGE-D1 antigen
  • NRAGE

Background

Neurotrophin receptor-interacting MAGE homolog (NRAGE, also known as MAGE-D1 or Dlxin-1) is a ubiquitously expressed cytosolic protein that is a member of the melanoma-associated antigen (MAGE) family. The 778 aa human NRAGE protein contains a segment with 22 repeats of a W(P/Q)xPxx motif, followed by a MAGE domain. In the brain, NRAGE interacts with p75NTR, blocks cell cycle progression and facilitates NGF-dependent apoptosis. Over amino acids 73‑254, human NRAGE shares 87% and 73% aa identity with mouse and rat NRAGE, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MAGED1
Uniprot