Hello, does this product (NB300-251) only recognize the cleavage Notch1?

Name: Notch-1 Antibody
Brand: Novus Biologicals
SKU: AF3647
Price: 459 USD

Question

Accepted Answer

This antibody with recognize full length Notch1 if it is in the mix, as well as the cleaved product.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	WB, Flow, ChIP, CyTOF-ready, ICC/IF
Clonality	Polyclonal
Host	Sheep
Conjugate	Unconjugated
Concentration	LYOPH

Catalog# & Conjugate	Size	Price
Biotin BAF3647

Catalog# & Formulation	Size	Price
Carrier Free AF3647-SP

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF3647

Notch-1 Antibody

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Publications for Notch-1 Antibody (AF3647)(10)

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Bioinformatics

Immunogen	E. coli-derived recombinant human Notch‑1 Gly2428-Lys2556 Accession # P46531
Specificity	Detects the intracellular domain (ICD) of human Notch-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) Notch-2 ICD, rhNotch-3 ICD, and rhNotch-4 ICD is observed.
Source	N/A
Isotype	IgG
Clonality	Polyclonal
Host	Sheep
Gene	NOTCH1
Purity Statement	Antigen Affinity-purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.2 mg/mL in sterile PBS.

Publications using AF3647	Applications	Species
Rajeh, A;Cornman, HL;Gupta, A;Szeto, MD;Kambala, A;Oladipo, O;Parthasarathy, V;Deng, J;Wheelan, S;Pritchard, T;Kwatra, MM;Semenov, YR;Gusev, A;Yegnasubramanian, S;Kwatra, SG; Somatic mutations reveal hyperactive Notch signaling and racial disparities in prurigo nodularis medRxiv : the preprint server for health sciences 2023-09-26 [PMID: 37808834] (IHC, Human)	IHC	Human
Huanhuan Joyce Chen, Asaf Poran, Arun M. Unni, Sarah Xuelian Huang, Olivier Elemento, Hans-Willem Snoeck et al. Generation of pulmonary neuroendocrine cells and SCLC-like tumors from human embryonic stem cells Journal of Experimental Medicine 3/4/2019 [PMID: 30737256]
J. Konen, E. Summerbell, B. Dwivedi, K. Galior, Y. Hou, L. Rusnak et al. Image-guided genomics of phenotypically heterogeneous populations reveals vascular signalling during symbiotic collective cancer invasion Nature Communications 5/12/2017 [PMID: 28497793]
Kai‐Yuan Chen, Tara Srinivasan, Kuei‐Ling Tung, Julio M Belmonte, Lihua Wang, Preetish Kadur Lakshminarasimha Murthy et al. A Notch positive feedback in the intestinal stem cell niche is essential for stem cell self‐renewal Molecular Systems Biology 4/30/2017 [PMID: 28455349]
SC Hrstka, X Li, TJ Nelson NOTCH1-dependent nitric oxide signaling deficiency in hypoplastic left heart syndrome revealed through patient-specific phenotypes detected in bioengineered cardiogenesis Stem Cells, 2017-03-05;0(0):. 2017-03-05 [PMID: 28142228] (ICC, Western Blot, Human)	ICC, Western Blot	Human
Yang Luo, Erica Y. Jacobs, Todd M. Greco, Kevin D. Mohammed, Tommy Tong, Sarah Keegan et al. HIV–host interactome revealed directly from infected cells Nature Microbiology 7/5/2016 [PMID: 27375898]
A dual molecular analogue tuner for dissecting protein function in mammalian cells Nat Commun, 2016-05-27;7(0):11742. 2016-05-27 [PMID: 27230261] (Western Blot, Human)	Western Blot	Human
Praveen Ballabh AMPA-Kainate Receptor Inhibition Promotes Neurologic Recovery in Premature Rabbits with Intraventricular Hemorrhage J. Neurosci., 2016-03-16;36(11):3363-77. 3/16/2016 [PMID: 26985043]
Gogoi D, Dar A, Chiplunkar S Involvement of Notch in activation and effector functions of gammadelta T cells. J Immunol, 2014-01-31;192(5):2054-62. 2014-01-31 [PMID: 24489102] (Flow Cytometry, Human)	Flow Cytometry	Human
Serge Camelo, William Raoul, Sophie Lavalette, Bertrand Calippe, Brunella Cristofaro, Olivier Levy et al. Delta-like 4 inhibits choroidal neovascularization despite opposing effects on vascular endothelium and macrophages Angiogenesis 12/1/2012 [PMID: 22869002]

Gene Symbol	NOTCH1
Uniprot	P46531()