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NKX2.2 Antibody (NX2/1524) [CoraFluor™ 1]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, IHC, MA, CyTOF-ready
Clone
NX2/1524
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-54323AF350
Alexa Fluor 405
NBP2-54323AF405
Alexa Fluor 488
NBP2-54323AF488
Alexa Fluor 532
NBP2-54323AF532
Alexa Fluor 594
NBP2-54323AF594
Alexa Fluor 647
NBP2-54323AF647
Alexa Fluor 700
NBP2-54323AF700
Alexa Fluor 750
NBP2-54323AF750
Allophycocyanin
NBP2-54323APC
Biotin
NBP2-54323B
DyLight 350
NBP2-54323UV
DyLight 405
NBP2-54323V
DyLight 488
NBP2-54323G
DyLight 550
NBP2-54323R
DyLight 594
NBP2-54323DL594
DyLight 650
NBP2-54323C
DyLight 680
NBP2-54323FR
DyLight 755
NBP2-54323IR
FITC
NBP2-54323F
HRP
NBP2-54323H
Janelia Fluor 525
NBP2-54323JF525
Janelia Fluor 549
NBP2-54323JF549
Janelia Fluor 646
NBP2-54323JF646
Janelia Fluor 669
NBP2-54323JF669
PE
NBP2-54323PE
PerCP
NBP2-54323PCP
Unconjugated
NBP2-53207-100ug
mFluor Violet 450 SE
NBP2-54323MFV450
mFluor Violet 500 SE
NBP2-54323MFV500
mFluor Violet 610 SE
NBP2-54323MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-53207-100ug
Azide and BSA Free
NBP2-54323-100ug
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NKX2.2 Antibody (NX2/1524) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Recombinant fragment (around aa1-119) of human NKX2.2 protein (exact sequence is proprietary) (Uniprot: O95096)
Localization
Nuclear
Marker
Neuroendocrine & Ewing's Sarcoma Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NKX2-2
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Protein Array
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for NKX2.2 Antibody (NX2/1524) [CoraFluor™ 1]

  • Homeobox protein NK-2 homolog B
  • NK-2 (Drosophila) homolog B
  • NK2 homeobox 2
  • NK2 transcription factor related, locus 2 (Drosophila)
  • NK2 transcription factor related, locus 2
  • NK2 transcription factor-like protein B
  • NKX2.2
  • NKX2.2homeobox protein Nkx-2.2
  • NKX2B
  • NKX2BNK-2 homolog B

Background

Nkx2.2, or Homeobox protein Nkx-2.2, consists of a 273 amino acid isoform that is 30 kDa, and is involved in controlling and maintaining the expression of genes within pancreatic endocrine cells and axons. Current research is being conducted on the relationship between Nkx2.2 and a variety of diseases and disorders, including glioma, neuronitis, maturity-onset diabetes, pancreatitis, Partington syndrome, sarcoma, gastrointestinal neuroendocrine tumor, acute lymphoblastic leukemia, multiple sclerosis, and carcinoid tumors. The protein has been linked to the Wnt, Hedgehog, and notch pathways, as well as regulation of beta-cell development and gene expression. NKx2.2 interacts with several other proteins, including OLIG2, HDAC1, SIN3A, FOXA2, and FOXO1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

ICC/IF Video Protocol

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Bioinformatics

Gene Symbol NKX2-2