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NKCC2/SLC12A1 Antibody

Images

 
Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP3-12228] - Rat stomach-NBP3-12228. 1:50 dilution in IHC blocking buffer. DAB (brown) staining and hematoxylin QS (blue) counterstrain. 40x magnification on leica ...read more
Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP3-12228] - Rat stomach. 1:150 dilution IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 10X, 40X magnification.

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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NKCC2/SLC12A1 Antibody Summary

Immunogen
Synthetic peptide taken within amino acid region 30-60 on NKCC2 protein.
Clonality
Polyclonal
Host
Rabbit
Gene
SLC12A1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot 1:500

Packaging, Storage & Formulations

Storage
Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Contains Tris, HCl/glycine buffer (pH 7.4-7.8), 30% glycerol, 0.5% BSA, along with cryo-protective agents, and HEPES (exact storage and stabilization buffer information is proprietary).
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for NKCC2/SLC12A1 Antibody

  • BSC1
  • Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2
  • Kidney-specific Na-K-Cl symporter
  • MGC48843
  • NKCC2
  • NKCC2A variant A
  • NKCC2Na-K-2Cl cotransporter
  • SLC12A1
  • solute carrier family 12 (sodium/potassium/chloride transporters), member 1
  • solute carrier family 12 member 1

Background

The sodium-potassium-chloride cotransporter isoform 2 is kidney-specific and is found on the apical membrane of the thick ascending limb of Henle's loop and the macula densa. It accounts for most of the NaCl resorption with the stoichiometry of 1Na:1K:2Cl and is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NKCC2/SLC12A1 Antibody (NBP3-12228) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SLC12A1