![Immunohistochemistry-Paraffin: Nicotinic Acetylcholine Receptor beta 4 Antibody [NBP1-20901] - (2.5ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-Receptor-beta-4-Antibody-Immunohistochemistry-Paraffin-NBP1-20901-img0001.jpg "Immunohistochemistry-Paraffin: Nicotinic Acetylcholine Receptor beta 4 Antibody [NBP1-20901] - (2.5ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-DYRLTWNSSRYEGVN corresponding to internal region according to NP_000741.1. |
| Epitope | C terminus |
| Specificity | The immunizing peptide represents part of the first exrracellular domain (aa22-236). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | CHRNB4 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Western blot: Preliminary experiments gave an approx 40kDa band in human testis lysates after 0.2ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.4kDa according to NP_000741.1. The 40kDa band was successfully blocked by incubation with the immunizing peptide. IHC: Paraffin embedded Human Uterus. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NBP1-20901 | Applications | Species |
|---|---|---|
| Liang Y, Salas R, Marubio L et al. Functional polymorphisms in the human beta4 subunit of nicotinic acetylcholine receptors. Neurogenetics 2005-02-01 [PMID: 15742216] |
Secondary Antibodies |
Isotype Controls |
Research Areas for Nicotinic Acetylcholine Receptor beta 4 Antibody (NBP1-20901)Find related products by research area.
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| Gene Symbol | CHRNB4 |
| Entrez |
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| Uniprot |
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