Recombinant Human Neuregulin-1 beta 1/NRG1 beta 1 Protein Summary
Description |
A single non-glycosylated polypeptide chain containing 65 amino acids corresponding to Neuregulin-1 beta 1/NRG1 beta 1 Source: E. coli Uniprot ID: Q02297 Amino Acid Sequence: SHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCPNEF TGDRCQNYVM ASFYKHLGIE FMEAE |
Details of Functionality |
Neuregulin-1 beta 1/NRG1 beta 1 protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x 10^6 IU/mg. |
Source |
E. coli |
Protein/Peptide Type |
Recombinant Protein |
Gene |
NRG1 |
Purity |
> 97 % pure by SDS-PAGE and HPLC |
Endotoxin Note |
Less than 0.1 EU/ug of Neuregulin-1 beta 1/NRG1 beta 1 as determined by LAL method. |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
7.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Buffer |
Lyophilized from a 0.2 um filtered solution in 1 x PBS, pH 7.4. |
Preservative |
5% Trehalose |
Concentration |
LYOPH |
Purity |
> 97 % pure by SDS-PAGE and HPLC |
Reconstitution Instructions |
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
Notes
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
Alternate Names for Recombinant Human Neuregulin-1 beta 1/NRG1 beta 1 Protein
Background
Neuregulin-1 (NRG1) is one of four Neuregulin proteins that contain an EGF-like domain that mediates their interaction with ErbB3 or ErbB4 to trigger intracellular signaling. Multiple transmembrane or soluble forms of NRG1 can be generated through alternative splicing, the use of alternative promoters, or proteolytic shedding. NRG1 isoforms play important roles during development of both the nervous system and the heart. They regulate neurotransmitter receptors expression in neurons and at the neuromuscular junction, and promote the differentiation and development of Schwann cells from neural crest stem cells.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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