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Human Neprilysin/CD10 ELISA Kit (Colorimetric)

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ELISA: Human Neprilysin/CD10 ELISA Kit (Colorimetric) [NBP2-62866] - Samples were spiked with high concentrations of Human Neprilysin/CD10 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human Neprilysin/CD10 ELISA Kit (Colorimetric) [NBP2-62866] - Samples were spiked with high concentrations of Human Neprilysin/CD10 and diluted with Reference Standard & Sample Diluent to produce samples with ...read more
ELISA: Human Neprilysin/CD10 ELISA Kit (Colorimetric) [NBP2-62866] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL

Order Details

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Human Neprilysin/CD10 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human NEP in samples. No significant cross-reactivity or interference between Human NEP and analogues was observed.
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.52%
Intra-Assay
CV% < 5.41%
Spike Recovery
94-112%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
MME

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Neprilysin/CD10 ELISA Kit (Colorimetric)

  • Atriopeptidase
  • CALLA
  • CALLAmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase)
  • CD10 antigen
  • CD10
  • CD10)
  • CD10membrane metallo-endopeptidase variant 1
  • Common acute lymphocytic leukemia antigen
  • DKFZp686O16152
  • EC 3.4.24
  • EC 3.4.24.11
  • Enkephalinase
  • EPN
  • Leu-19
  • membrane metallo-endopeptidase variant 2
  • membrane metallo-endopeptidase
  • MGC126681
  • MGC126707
  • MME
  • NEPmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA
  • Neprilysin
  • Neutral Endopeptidase 24.11
  • Neutral endopeptidase
  • NKH1
  • SFE
  • Skin fibroblast elastase

Background

CD10, also known as the Common Acute Lymphocytic Leukemia Antigen (CALLA), is a cell surface enzyme with neutral metalloendopeptidase activity which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt's, and follicular germinal center lymphomas, immature B cells with in adult bone marrow and on cells from patients with chronic myelocytic leukemia (CML). CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product PDFs

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  Human Neprilysin/CD10 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol MME