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Nectin-2/CD112 Antibody (JG39-64)

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Western Blot: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852] - Analysis of Nectin 2 on K562 (1), MCF-7 (2) and SK-OV-3 (3) cells lysates using anti-Nectin 2 antibody at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852] - ICC staining Nectin 2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, ...read more
Immunohistochemistry: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852] - Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Nectin 2 antibody. Counter stained with hematoxylin.
Flow Cytometry: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852] - Flow cytometric analysis of LOVO cells with Nectin 2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation ...read more
Immunocytochemistry/ Immunofluorescence: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852] - ICC staining Nectin 2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, ...read more
Immunohistochemistry: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852] - Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Nectin 2 antibody. Counter stained with hematoxylin.
Immunohistochemistry: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852] - Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Nectin 2 antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
JG39-64
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

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Nectin-2/CD112 Antibody (JG39-64) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Recombinant protein within Human Nectin-2/CD112 aa 370-538 / 538. (SwissProt: Q92692 Human; SwissProt: P32507 Mouse; SwissProt: Q5FVC5 Rat)
Localization
Cell membrane. Membrane.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
NECTIN2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
Theoretical MW
58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Nectin-2/CD112 Antibody (JG39-64)

  • CD112 antigen
  • CD112
  • Herpes virus entry mediator B
  • Herpesvirus entry mediator B
  • herpesvirus entry protein B
  • HVEB
  • HVEBpoliovirus receptor-like 2
  • MPH
  • nectin 2
  • Nectin2
  • Nectin-2
  • poliovirus receptor-related 2 (herpesvirus entry mediator B)
  • poliovirus receptor-related protein 2
  • PRR2
  • PRR2nectin-2
  • PVRL2
  • PVRR2poliovirus receptor related 2

Background

Nectin 2 encodes a single-pass type I membrane glycoprotein with two Ig-like C2-type domains and an Ig-like V-type domain. This protein is one of the plasma membrane components of adherens junctions. It also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in this gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NECTIN2