Novus Biologicals products are now on bio-techne.com

Recombinant Human NDUFS2 His Protein

Images

 
SDS-Page: NDUFS2 Protein [NBP2-23265]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.25 mg/ml

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Recombinant Human NDUFS2 His Protein Summary

Description
A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 77-463 of Human NDUFS2

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSVKNITLN FGPQHPAAHG VLRLVMELSG EMVRKCDPHI GLLHRGTEKL IEYKTYLQAL PYFDRLDYVS MMCNEQAYSL AVEKLLNIRP PPRAQWIRVL FGEITRLLNH IMAVTTHALD LGAMTPFFWL FEEREKMFEF YERVSGARMH AAYIRPGGVH QDLPLGLMDD IYQFSKNFSL RLDELEELLT NNRIWRNRTI DIGVVTAEEA LNYGFSGVML RGSGIQWDLR KTQPYDVYDQ VEFDVPVGSR GDCYDRYLCR VEEMRQSLRI IAQCLNKMPP GEIKVDDAKV SPPKRAEMKT SMESLIHHFK LYTEGYQVPP GATYTAIEAP KGEFGVYLVS DGSSRPYRCK IKAPGFAHLA GLDKMSKGHM LADVVAIIGT QDIVFGEVDR

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
NDUFS2
Purity
>80%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Application Notes
Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
Theoretical MW
46.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
Preservative
No Preservative
Concentration
0.25 mg/ml
Purity
>80%, by SDS-PAGE

Alternate Names for Recombinant Human NDUFS2 His Protein

  • CI-49
  • CI-49kD
  • complex 1, mitochondrial respiratory chain, 49-KD subunit
  • complex I 49kDa subunit
  • Complex I-49kD
  • EC 1.6.5.3
  • EC 1.6.99.3
  • EC 1.6.99.5
  • NADH dehydrogenase (ubiquinone) Fe-S protein 2 (49kD) (NADH-coenzyme Qreductase)
  • NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Qreductase)
  • NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial
  • NADH-ubiquinone oxidoreductase 49 kDa subunit
  • NADH-ubiquinone oxidoreductase NDUFS2 subunit

Background

NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial isoform 1 precursor, also known as NDUFS2, is a 463 amino acid protein that is suggested to be required for catalytic activity. Defects in NDUFS2 are the cause of complex I mitochondrial respiratory chain deficiency, which is characterized by a variety of symptoms including liver failure, cardiomyopathy and neurodegeneration. Located in the mitochondrial inner membrane, mitochondrial complex I is the first and largest enzyme in the electron transport chain of oxidative phosphorylation. Recombinant human NDUFS2 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-31142
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP1-33074
Species: Hu, Mu, Ze
Applications: IHC, IHC-P, KO, WB
NBP1-49846
Species: Hu
Applications: IHC, IHC-P, PEP-ELISA, WB
NBP1-85620
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-89026
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, WB
H00004722-M02
Species: Hu
Applications: ELISA, IHC, IHC-P, IP, KD, WB
NBP1-84475
Species: Hu, Mu
Applications: IHC, IHC-P, WB
NBP1-49831
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, PEP-ELISA, WB
NBP1-77397
Species: Hu, Mu
Applications: Flow, IB, ICC/IF, IHC, IHC-P, KD, Simple Western, WB
QET00B
Species: Hu
Applications: ELISA
NBP1-59560
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-03825
Species: Ca, Hu, Pm, Mu, Pm, Rt
Applications: Flow, IHC, IHC-P, WB
AF3014
Species: Hu
Applications: Block, IHC, WB
NBP2-93398
Species: Hu, Mu, Rt
Applications: WB
NBP2-33710
Species: Hu
Applications: ICC/IF, IHC, IHC-P
H00004704-M01
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, S-ELISA, WB
NBP3-03538
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KO, WB
NBP1-92172
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-93264
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-23265
Species: Hu
Applications: PAGE

Publications for NDUFS2 Protein (NBP2-23265) (0)

There are no publications for NDUFS2 Protein (NBP2-23265).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for NDUFS2 Protein (NBP2-23265) (0)

There are no reviews for NDUFS2 Protein (NBP2-23265). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for NDUFS2 Protein (NBP2-23265) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional NDUFS2 Products

Research Areas for NDUFS2 Protein (NBP2-23265)

Find related products by research area.

Blogs on NDUFS2

There are no specific blogs for NDUFS2, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human NDUFS2 His Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol NDUFS2