E. coli reactivity reported in scientific literature (PMID: 30323356). Use in Mouse reported in scientific literature (PMID:32014252).
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified
Background
New Delhi metallo-beta-lactamase is an enzyme that predisposes bacteria to become antibiotic resistant. In Pakistan, multi-drug resistant bacteria are blamed for a higher than usual incidence of hospital infections. NDM1 has an interesting conformational design, in that it has a large active site which allows it to react with multiple substrates, and which changes distinctly after substrates bind to it. Therapies against NDM1 in tandem with antibiotic therapies are being postulated as the best potential defense against antibiotic resistance. However, the ability to identify NDM1 prevalence is also critical. Identification of colonized individuals by immunodetection assays could be a critical step in isolation and treatment.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for NDM-1 Antibody (NBP1-77688)(7)
We have publications tested in 3 confirmed species: Mouse, Bacteria, E. coli.
FAQs for NDM-1 Antibody (NBP1-77688). (Showing 1 - 1 of 1 FAQs).
If I want to detect the presence of NDM-1 protein in our isolates (Acinetobacter), would you please let me know if I can used this Antibody via WB?
Here is the information on the immunogen for this antibody: A synthetic peptide made to a C-terminal region of the E. coli bla-NDM-1 protein (within residues 100-158). [NCBI BAJ10873] We have confirmed this antibody to work in WB, but I do not believe the lab has tested in Acinetobacter specifically. If the protein is expressed in those samples and should have high similarity with the sequence provided for the immunogen then most likely it should work well, but I cannot say for certain since we haven't directly tested.
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![Western Blot: NDM-1 Antibody [NBP1-77688] - Analysis of NDM1 in partial recombinant NDM1 protein.](http://images.novusbio.com/fullsize/NDM-1-Antibody-Western-Blot-NBP1-77688-img0001.jpg "Western Blot: NDM-1 Antibody [NBP1-77688] - Analysis of NDM1 in partial recombinant NDM1 protein.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")