Reactivity | HuSpecies Glossary |
Applications | IHC |
Clonality | Polyclonal |
Host | Sheep |
Conjugate | Unconjugated |
Concentration | LYOPH |
Immunogen | E. coli-derived recombinant human NCOR2 Lys2268-Arg2449 Accession # Q9Y618 |
Specificity | Detects human NCOR2 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human NCOR1 is observed. |
Source | N/A |
Isotype | IgG |
Clonality | Polyclonal |
Host | Sheep |
Gene | NCOR2 |
Purity Statement | Antigen Affinity-purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
Preservative | No Preservative |
Concentration | LYOPH |
Reconstitution Instructions | Sterile PBS to a final concentration of 0.2 mg/mL. |
NCOR2 (Nuclear receptor Co-Repressor 2; also TRAC-2, SMRT alpha and CTG26) is a 265-275 kDa member of the N-CoR family of molecules. NCOR2 is a transcriptional repressor. It forms a complex with Class I and II HDACs (particularly HDAC3) plus TBL1and GPS2, and interacts with a ligand-independent T3R:RXR heterodimer bound to select gene promoters. The HDACs deacetylate lysines in histones, promoting gene repressor binding. Human NCOR2 is 2525 amino acids (aa) in length. It possesses an N-terminal coiled-coil region (aa 174-215), one DNA-binding SANT domain (aa 427-478), a second coiled-coil region (aa 522-561), and a second SANT domain (aa 610-661). There are poly-Gly, -Gln, -Pro and -Lys motifs, and at least five acetylated lysine residues. There are also at least 31 Ser, three Thr, and one Tyr utilized phosphorylation sites that may regulate complex dissociation. There are also multiple splice variants. One isoform (SMRT tau ) contains a deletion of aa 724-740 and 2361-2406; a second isoform (SMRTe) contains only a deletion of aa 724-740; a third isoform (hSMRT) shows a deletion of aa 1034-1041; and a fourth isoform (TRAC-1) possesses an alternative start site at Met 1711 coupled to a deletion of aa 2361-2406. Over aa 2268-2449, human NCOR2 shares 95% aa identity with mouse NCOR2.
Secondary Antibodies |
Isotype Controls |
Muscular Dystrophy Regulation: Ezh2, NCoR-1 Over the years muscular dystrophies have become a popular area of research. These are a group of inherited disorders that involve an increase in muscle weakness over time. These disorders greatly decrease the quality of life and there are no known cur... Read full blog post. |
NCOR & EZH2 in Muscular Dystrophy Over the years muscular dystrophies have become a popular area of research. These are a group of inherited disorders that involve an increase in muscle weakness over time. These disorders greatly decrease the quality of life and there are no known cur... Read full blog post. |
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