![Western Blot: Napsin A Antibody (NAPSA/1238) - BSA Free [NBP2-54383] - K562, HEK293 andA549 Cell Lysates Using Napsin-A Monoclonal Antibody (NAPSA/1238)](http://images.novusbio.com/fullsize/Napsin-A-Antibody-NAPSA-1238-BSA-Free-Western-Blot-NBP2-54383-img0002.jpg "Western Blot: Napsin A Antibody (NAPSA/1238) - BSA Free [NBP2-54383] - K562, HEK293 andA549 Cell Lysates Using Napsin-A Monoclonal Antibody (NAPSA/1238)")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, MA, CyTOF-ready |
| Clone | NAPSA/1238 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS WITHOUT BSA. Also available at 200 ug/ml WITH BSA & azide (NBP2-45244). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant human Napsin A protein fragment (around aa189-299) (exact sequence is proprietary) (Uniprot: O96009) |
| Localization | Cytoplasmic |
| Marker | Lung Adenocarcinoma Marker |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NAPSA |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. Western Blot: 1-2ug/ml for 60 minutes at RT. |
| Theoretical MW | 37 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Napsin A Antibody (NBP2-54383)Find related products by research area.
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| Gene Symbol | NAPSA |
![Immunohistochemistry-Paraffin: Napsin A Antibody (NAPSA/1238) - BSA Free [NBP2-54383] - Formalin-fixed, paraffin-embedded human Lung Adenocarcinoma stained with Napsin-A Monoclonal Antibody (NAPSA/1238).](http://images.novusbio.com/fullsize/Napsin-A-Antibody-NAPSA-1238-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54383-img0001.jpg "Immunohistochemistry-Paraffin: Napsin A Antibody (NAPSA/1238) - BSA Free [NBP2-54383] - Formalin-fixed, paraffin-embedded human Lung Adenocarcinoma stained with Napsin-A Monoclonal Antibody (NAPSA/1238).")
![Protein Array: Napsin A Antibody (NAPSA/1238) - BSA Free [NBP2-54383] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Napsin A Antibody (NAPSA/1238). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Napsin-A-Antibody-NAPSA-1238-BSA-Free-Protein-Array-NBP2-54383-img0003.jpg "Protein Array: Napsin A Antibody (NAPSA/1238) - BSA Free [NBP2-54383] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Napsin A Antibody (NAPSA/1238). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.")
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")