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MYLK2 Antibody

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Western Blot: MYLK2 Antibody [NBP2-11574] - (0.03ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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MYLK2 Antibody Summary

Immunogen
Peptide with sequence C-KDTKNMEAKKLSKD corresponding to internal region according to NP_444253.3, NP_444254.3 , NP_444255.3, NP_444256.3.
Epitope
CKDTKNMEAKKLSKD
Specificity
This antibody is expected to recognise all reported isoforms (NP_444253.3; NP_444254.3 ; NP_444255.3 and NP_444256.3).
Predicted Species
Mouse (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MYLK2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.01 - 0.03 ug/ml
Application Notes
WB: Approx. 150 kDa band observed in human colon and lung lysates (calculated MW of 197 kDa band according to NP_444256.3).
Publications
Read Publication using NBP2-11574.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MYLK2 Antibody

  • EC 2.7.11
  • EC 2.7.11.18
  • KMLC
  • MLCK2
  • MLCK2MLCK
  • MYLK2
  • myosin light chain kinase 2
  • myosin light chain kinase 2, skeletal/cardiac muscle
  • skeletal muscle
  • skMLCK

Background

MYLK2 encodes a myosin light chain kinase, a calcium/calmodulin dependent enzyme, that is exclusively expressed in adult skeletal muscle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MYLK2 Antibody (NBP2-11574)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MYLK2
Entrez