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MUC3 Antibody (MUC3/1154) [Janelia Fluor® 585]

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Product Details

Summary
Reactivity Hu, Rt(-)Species Glossary
Applications Flow, ICC/IF, IHC, CyTOF-ready, ICC/IF
Clone
MUC3/1154
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 585

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-47687AF350
Alexa Fluor 405
NBP2-47687AF405
Alexa Fluor 488
NBP2-47687AF488
Alexa Fluor 532
NBP2-47687AF532
Alexa Fluor 594
NBP2-47687AF594
Alexa Fluor 647
NBP2-47687AF647
Alexa Fluor 700
NBP2-47687AF700
Alexa Fluor 750
NBP2-47687AF750
Allophycocyanin
NBP2-47687APC
Allophycocyanin/Cy7
NBP2-47687APCCY7
Biotin
NBP2-47687B
CoraFluor 1
NBP2-47687CL1
DyLight 350
NBP2-47687UV
DyLight 405
NBP2-47687V
DyLight 488
NBP2-47687G
DyLight 550
NBP2-47687R
DyLight 594
NBP2-47687DL594
DyLight 650
NBP2-47687C
DyLight 680
NBP2-47687FR
DyLight 755
NBP2-47687IR
FITC
NBP2-47687F
HRP
NBP2-47687H
Janelia Fluor 525
NBP2-47687JF525
Janelia Fluor 549
NBP2-47687JF549
Janelia Fluor 635
NBP2-47687JF635
Janelia Fluor 646
NBP2-47687JF646
Janelia Fluor 669
NBP2-47687JF669
PE
NBP2-47687PE
PE/Atto594
NBP2-47687PEATT594
PE/Cy5.5
NBP2-47687PECY55
PE/Cy7
NBP2-47687PECY7
PerCP
NBP2-47687PCP
Unconjugated
NBP2-44434-0.1mg
mFluor Violet 450 SE
NBP2-47687MFV450
mFluor Violet 500 SE
NBP2-47687MFV500
mFluor Violet 610 SE
NBP2-47687MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-44434-0.1mg
Azide and BSA Free
NBP2-47687-0.1mg
IHC-Prediluted
NBP2-48415
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MUC3 Antibody (MUC3/1154) [Janelia Fluor® 585] Summary

Immunogen
A synthetic peptide of 35 amino acids, SIB35 (C-HSTPSFTSSITTTETTSHSTPSFTSSITTTETTS), which contains two of the MUC3 tandem repeats, coupled to KLH.
Localization
Cytoplasmic and cell surface
Specificity
It recognizes a protein of HMW, identified as mucin 3 glycoprotein (MUC3). This monoclonal antibody shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC2. The Mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs) encoded by 60 base pairs (Mucin 1), 69 base pairs (Mucin 2) and 51 base pairs (Mucin 3). The number of repeats is highly polymorphic and varies among different alleles. Mucin 1 proteins are expressed as type I membrane proteins in addition to secreted forms. Mucin 1 is aberrantly expressed in epithelial tumors including breast carcinomas. Mucin 2 coats the epithelia of the intestines and airways and is associated with colonic tumors. Mucin 3 is a major component of various mucus gels and is broadly expressed in normal and tumor cells.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MUC3A
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Does not react with Rat.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for MUC3 Antibody (MUC3/1154) [Janelia Fluor® 585]

  • MUC-3A
  • mucin 3, intestinal
  • mucin 3A, cell surface associated
  • mucin 3A, intestinal
  • mucin-3A

Background

The mucin genes encode epithelial glycoproteins, some of which are secreted and some membrane bound. Each of the genes contains at least one large domain of tandemly repeated sequence that encodes the peptide sequence rich in serine and/or threonine residues, which carries most of the O-linked glycosylation (Gendler and Spicer, 1995 (PubMed 7778880)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MUC3 Antibody
(MUC3/1154) [Janelia ...
NBP2-47687JF585
MUC3 Antibody (MUC3/1154) [Janelia Fluor® 585]
Species: Hu, Rt(-)
Applications: Flow, ICC/IF, IHC, CyTOF-ready, ICC/IF

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Bioinformatics

Gene Symbol MUC3A