![Western Blot: MPP3 Antibody (4D7) [H00004356-M01] - Analysis of MPP3 expression in transfected 293T cell line by MPP3 monoclonal antibody (M01), clone 4D7.Lane 1: MPP3 transfected lysate(66.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MPP3-Antibody-4D7-Western-Blot-H00004356-M01-img0006.jpg "Western Blot: MPP3 Antibody (4D7) [H00004356-M01] - Analysis of MPP3 expression in transfected 293T cell line by MPP3 monoclonal antibody (M01), clone 4D7.Lane 1: MPP3 transfected lysate(66.2 KDa).Lane 2: Non-transfected lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, KD |
| Clone | 4D7 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | MPP3 (AAH56865, 1 a.a. ~ 585 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPVLSEDSGLHETLALLTSQLRPDSNHKEEMGFLRDVFSEKSLSYLMKIHEKLRYYERQSPTPVLHSAVALAEDVMEELQAASVHSDERELLQLLSTPHLRAVLMVHDTVAQKNFDPVLPPLPDNIDEDFDEESVKIVRLVKNKEPLGATIRRDEHSGAVVVARIMRGGAADRSGLVHVGDELREVNGIAVLHKRPDEISQILAQSQGSITLKIIPATQEEDRLKESKVFMRALFHYNPREDRAIPCQEAGLPFQRRQVLEVVSQDDPTWWQAKRVGDTNLRAGLIPSKGFQERRLSYRRAAGTLPSPQSLRKPPYDQPCDKETCDCEGYLKGHYVAGLRRSFRLGCRERLGGSQEGKMSSGAESPELLTYEEVARYQHQPGERPRLVVLIGSLGARLHELKQKVVAENPQHFGVAVPHTTRPRKSHEKEGVEYHFVSKQAFEADLHHNKFLEHGEYKENLYGTSLEAIQAVMAKNKVCLVDVEPEALKQLRTSEFKPYIIFVKPAIQEKRKTPPMSPACEDTAAPFDEQQQEMAASAAFIDRHYGHLVDAVLVKEDLQGAYSQLKVVLEKLSKDTHWVPVSWVR |
| Specificity | MPP3 - membrane protein, palmitoylated 3 (MAGUK p55 subfamily member 3) |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | MPP3 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
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![Western Blot: MPP3 Antibody (4D7) [H00004356-M01] - Analysis of MPP3 over-expressed 293 cell line, cotransfected with MPP3 Validated Chimera RNAi ( Cat # H00004356-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MPP3 monoclonal antibody (M01), clone 4D7 (Cat # H00004356-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/MPP3-Antibody-4D7-Western-Blot-H00004356-M01-img0004.jpg "Western Blot: MPP3 Antibody (4D7) [H00004356-M01] - Analysis of MPP3 over-expressed 293 cell line, cotransfected with MPP3 Validated Chimera RNAi ( Cat # H00004356-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MPP3 monoclonal antibody (M01), clone 4D7 (Cat # H00004356-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.")
![Sandwich ELISA: MPP3 Antibody (4D7) [H00004356-M01] - Detection limit for recombinant GST tagged MPP3 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MPP3-Antibody-4D7-Sandwich-ELISA-H00004356-M01-img0005.jpg "Sandwich ELISA: MPP3 Antibody (4D7) [H00004356-M01] - Detection limit for recombinant GST tagged MPP3 is approximately 0.03ng/ml as a capture antibody.")
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")