Mouse Thrombopoietin Quantikine ELISA Kit, 2 Plate

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Thrombopoietin (Tpo), is a key regulator of megakaryocytopoiesis and thrombopoiesis. It is principally produced in the liver and is bound and internalized by the receptor Tpo R/c-mpl. Defects in the Tpo-Tpo R signaling pathway are associated with a variety of platelet disorders. The 353 amino acid (aa) human Tpo precursor is cleaved to yield the 332 aa mature protein. Mature human Tpo shares approximately 70% aa sequence homology with mouse and rat Tpo. It is an 80 - 85 kDa protein that consists of an N-terminal domain with homology to Erythropoietin (Epo) and a C-terminal domain that contains multiple N-linked and O-linked glycosylation sites. Tissue specific alternate splicing of human Tpo generates multiple isoforms with internal deletions, insertions, and/or C-terminal substitutions. Tpo promotes the differentiation, proliferation, and maturation of MK and their progenitors. Several other cytokines can promote these functions as well but only in cooperation with Tpo. Notably, IL-3 independently induces MK development, although its effects are restricted to early in the MK lineage. Tpo additionally promotes platelet production, aggregation, ECM adhesion, and activation. It is cleaved by platelet-derived thrombin following Arg191 within the C-terminal domain and subsequently at other sites upon extended digestion. Full length Tpo and shorter forms circulate in the plasma. The C-terminal domain is not required for binding to Tpo R or inducing MK growth and differentiation. Aside from its hematopoietic effects, Tpo is expressed in the brain where it promotes the apoptosis of hypoxia-sensitized neurons and inhibits neuronal differentiation by blocking NGF induced signaling.

Thrombopoietin receptor (Tpo R), also known as myeloproliferative leukemia protein (c-Mpl), is a type I transmembrane protein that is a member of the hematopoietin/cytokine receptor superfamily. As a consequence of alternative splicing, there are four identified mRNA variants. The functional receptor is encoded by the P isoform of mRNA. The human and mouse receptors share approximately 81% amino acid sequence identity. Tpo R is expressed at low levels in various cell types, including hematopoietic progenitor cells, megakaryocytes and platelets.

We have publications tested in 2 confirmed species: Mouse, Transgenic Mouse.

We have publications tested in 1 application: ELISA Capture.

Showing Publications 1 - 10 of 43. Show All 43 Publications. Collapse Publications.

Publications using MTP00	Applications	Species
Gaertner, F;Ishikawa-Ankerhold, H;Stutte, S;Fu, W;Weitz, J;Dueck, A;Nelakuditi, B;Fumagalli, V;van den Heuvel, D;Belz, L;Sobirova, G;Zhang, Z;Titova, A;Navarro, AM;Pekayvaz, K;Lorenz, M;von Baumgarten, L;Kranich, J;Straub, T;Popper, B;Zheden, V;Kaufmann, WA;Guo, C;Piontek, G;von Stillfried, S;Boor, P;Colonna, M;Clau beta , S;Schulz, C;Brocker, T;Walzog, B;Scheiermann, C;Aird, WC;Nerlov, C;Stark, K;Petzold, T;Engelhardt, S;Sixt, M;Hauschild, R;Rudelius, M;Oostendorp, RAJ;Iannacone, M;Heinig, M;Massberg, S; Plasmacytoid dendritic cells control homeostasis of megakaryopoiesis Nature 2024-07-01 [PMID: 38987596] (Transgenic Mouse)		Transgenic Mouse
Becker, IC;Wilkie, AR;Nikols, E;Carminita, E;Roweth, HG;Tilburg, J;Sciaudone, AR;Noetzli, LJ;Fatima, F;Couldwell, G;Ray, A;Mogilner, A;Machlus, KR;Italiano, JE; Cell cycle-dependent centrosome clustering precedes proplatelet formation Science advances 2024-06-21 [PMID: 38896608] (Mouse)		Mouse
Behrens, K;Kauppi, M;Viney, EM;Kueh, AJ;Hyland, CD;Willson, TA;Salleh, L;de Graaf, CA;Babon, JJ;Herold, MJ;Nicola, NA;Alexander, WS; Differential in vivo roles of Mpl cytoplasmic tyrosine residues in murine hematopoiesis and myeloproliferative disease Leukemia 2024-03-15 [PMID: 38491305] (Transgenic Mouse)		Transgenic Mouse
Holmes-Hampton, GP;Kumar, VP;Biswas, S;Stone, S;Sharma, NK;Legesse, B;Vercellino, J;Guha, C;Eichenbaum, G;Ghosh, SP; PEGylated thrombopoietin mimetic, JNJ?26366821 a novel prophylactic radiation countermeasure for acute radiation injury Scientific reports 2023-09-14 [PMID: 37709916] (Mouse)		Mouse
Becker, HJ;Ishida, R;Wilkinson, AC;Kimura, T;Lee, MSJ;Coban, C;Ota, Y;Tanaka, Y;Roskamp, M;Sano, T;Tojo, A;Kent, DG;Yamazaki, S; Controlling genetic heterogeneity in gene-edited hematopoietic stem cells by single-cell expansion Cell stem cell 2023-07-06 [PMID: 37385251] (Mouse)		Mouse
Kocatürk, B;Lee, Y;Nosaka, N;Abe, M;Martinon, D;Lane, ME;Moreira, D;Chen, S;Fishbein, MC;Porritt, RA;Franklin, BS;Noval Rivas, M;Arditi, M; Platelets exacerbate cardiovascular inflammation in a murine model of Kawasaki disease vasculitis JCI insight 2023-06-06 [PMID: 37279077] (Mouse)		Mouse
Jeong, SH;Park, JY;Ryu, YB;Kim, WS;Lee, IC;Kim, JH;Kim, D;Ha, JH;Lee, BW;Nam, J;Cho, KO;Kwon, HJ; Myristica fragrans Extract Inhibits Platelet Desialylation and Activation to Ameliorate Sepsis-Associated Thrombocytopenia in a Murine CLP-Induced Sepsis Model International journal of molecular sciences 2023-05-16 [PMID: 37240208] (Mouse)		Mouse
SY Heazlewood, T Ahmad, B Cao, H Cao, M Domingues, X Sun, CK Heazlewood, S Li, B Williams, M Fulton, JF White, T Nebl, CM Nefzger, JM Polo, BT Kile, F Kraus, MT Ryan, YB Sun, PFM Choong, SL Ellis, ML Anko, SK Nilsson High ploidy large cytoplasmic megakaryocytes are hematopoietic stem cells regulators and essential for platelet production Nature Communications, 2023-04-13;14(1):2099. 2023-04-13 [PMID: 37055407] (Mouse)		Mouse
HG Roweth, MW Malloy, GJ Goreczny, IC Becker, Q Guo, EA Mittendorf, JE Italiano, SS McAllister, EM Battinelli Pro-inflammatory megakaryocyte gene expression in murine models of breast cancer Science Advances, 2022-10-12;8(41):eabo5224. 2022-10-12 [PMID: 36223471] (Mouse)		Mouse
MGE Rommel, L Walz, F Fotopoulou, S Kohlscheen, F Schenk, C Miskey, L Botezatu, Y Krebs, IM Voelker, K Wittwer, T Holland-Le, Z Ivics, V von Messli, MAG Essers, MD Milsom, CK Pfaller, U Modlich Influenza A virus infection instructs hematopoiesis to megakaryocyte-lineage output Cell Reports, 2022-10-04;41(1):111447. 2022-10-04 [PMID: 36198277] (Mouse)		Mouse
JL Spivak, A Merchant, DM Williams, O Rogers, W Zhao, A Duffield, LS Resar, AR Moliterno, ZJ Zhao Thrombopoietin is required for full phenotype expression in a JAK2V617F transgenic mouse model of polycythemia vera PLoS ONE, 2020-06-01;15(6):e0232801. 2020-06-01 [PMID: 32479500] (Transgenic Mouse)		Transgenic Mouse
CD Wiley, S Liu, C Limbad, AM Zawadzka, J Beck, M Demaria, R Artwood, F Alimirah, JA Lopez-Domi, C Kuehnemann, SR Danielson, N Basisty, HG Kasler, TR Oron, PY Desprez, SD Mooney, BW Gibson, B Schilling, J Campisi, P Kapahi SILAC Analysis Reveals Increased Secretion of Hemostasis-Related Factors by Senescent Cells Cell Rep, 2019-09-24;28(13):3329-3337.e5. 2019-09-24 [PMID: 31553904] (Mouse)		Mouse
B Hurtado, M Trakala, P Ximénez-Em, A El Bakkali, D Partida, B Sanz-Casti, M Álvarez-Fe, M Maroto, R Sánchez-Ma, L Martínez, J Muñoz, P García de, M Malumbres Thrombocytopenia-associated mutations in Ser/Thr kinase MASTL deregulate actin cytoskeleton dynamics in platelets J. Clin. Invest., 2018-10-29;0(0):. 2018-10-29 [PMID: 30252678] (Mouse)		Mouse
Q Ma, C Zhu, W Zhang, N Ta, R Zhang, L Liu, D Feng, H Cheng, J Liu, Q Chen Mitochondrial PIP3-binding protein FUNDC2 supports platelet survival via AKT signaling pathway Cell Death Differ., 2018-05-21;0(0):. 2018-05-21 [PMID: 29786068] (Mouse)		Mouse
S Ding, M Wang, S Fang, H Xu, H Fan, Y Tian, Y Zhai, S Lu, X Qi, F Wei, G Sun, X Sun D-dencichine Regulates Thrombopoiesis by Promoting Megakaryocyte Adhesion, Migration and Proplatelet Formation Front Pharmacol, 2018-04-03;9(0):297. 2018-04-03 [PMID: 29666579] (Mouse)		Mouse
MB Alvarez, L Xu, P Childress, KA Maupin, SFG Mohamad, B Chitteti, E Himes, DJ Olivos Iii, YH Cheng, SJ Conway, EF Srour, MA Kacena Megakaryocyte and Osteoblast Interactions Modulate Bone Mass and Hematopoiesis Stem Cells Dev., 2018-05-15;0(0):. 2018-05-15 [PMID: 29631496] (Mouse)		Mouse
YX Tan, H Cui, LM Wan, F Gong, X Zhang, I Vlodavsky, JP Li Overexpression of heparanase in mice promoted megakaryopoiesis Glycobiology, 2018-05-01;0(0):. 2018-05-01 [PMID: 29471321] (ELISA Capture, Mouse)	ELISA Capture	Mouse
M Zingariell, L Sancillo, F Martelli, F Ciaffoni, M Marra, L Varricchio, RA Rana, C Zhao, JD Crispino, AR Migliaccio The thrombopoietin/MPL axis is activated in the Gata1(low) mouse model of myelofibrosis and is associated with a defective RPS14 signature Blood Cancer J, 2017-06-16;7(6):e572. 2017-06-16 [PMID: 28622305] (Mouse)		Mouse
Repercussion of Megakaryocyte-Specific Gata1 Loss on Megakaryopoiesis and the Hematopoietic Precursor Compartment PLoS ONE, 2016-05-06;11(5):e0154342. 2016-05-06 [PMID: 27152938] (Mouse)		Mouse
Bender M, Giannini S, Grozovsky R, Jonsson T, Christensen H, Pluthero F, Ko A, Mullally A, Kahr W, Hoffmeister K, Falet H Dynamin 2-dependent endocytosis is required for normal megakaryocyte development in mice. Blood, 2014-12-02;125(6):1014-24. 2014-12-02 [PMID: 25468568] (Mouse)		Mouse
Nakamura-Ishizu , Ayako, Takubo , Keiyo, Fujioka , Masato, Suda , Toshio Megakaryocytes are essential for HSC quiescence through the production of thrombopoietin. Biochem Biophys Res Commun, 2014-10-24;454(2):353-7. 2014-10-24 [PMID: 25451253] (Mouse)		Mouse
Greene T, Lyde R, Bailey S, Lambert M, Zhai L, Sabatino D, Camire R, Arruda V, Poncz M Apoptotic effects of platelet factor VIII on megakaryopoiesis: implications for a modified human FVIII for platelet-based gene therapy. J Thromb Haemost, 2014-11-04;12(12):2102-12. 2014-11-04 [PMID: 25287191] (Mouse)		Mouse
Molina-Ortiz P, Polizzi S, Ramery E, Gayral S, Delierneux C, Oury C, Iwashita S, Schurmans S Rasa3 controls megakaryocyte Rap1 activation, integrin signaling and differentiation into proplatelet. PLoS Genet, 2014-06-26;10(6):e1004420. 2014-06-26 [PMID: 24967784] (Mouse)		Mouse
Josefsson , Emma C, Burnett , Deborah, Lebois , Marion, Debrincat , Marlyse, White , Michael, Henley , Katya J, Lane , Rachael, Moujalled , Diane, Preston , Simon P, O'Reilly , Lorraine, Pellegrini , Marc, Metcalf , Donald, Strasser , Andreas, Kile , Benjamin Platelet production proceeds independently of the intrinsic and extrinsic apoptosis pathways. Nat Commun, 2014-03-17;5(0):3455. 2014-03-17 [PMID: 24632563] (Mouse)		Mouse
Grover , Amit, Mancini , Elena, Moore , Susan, Mead , Adam J, Atkinson , Deborah, Rasmussen , Kasper D, O'Carroll , Donal, Jacobsen , Sten Eir, Nerlov , Claus Erythropoietin guides multipotent hematopoietic progenitor cells toward an erythroid fate. J Exp Med, 2014-02-03;211(2):181-8. 2014-02-03 [PMID: 24493804] (Mouse)		Mouse
Hong S, Dvorak-Ewell M, Stevens H, Barish G, Castro G, Nofsinger R, Frangos J, Shoback D, Evans R Rescue of a primary myelofibrosis model by retinoid-antagonist therapy. Proc Natl Acad Sci U S A, 2013-11-04;110(47):18820-5. 2013-11-04 [PMID: 24191050] (Mouse)		Mouse
Zhang L, Orban M, Lorenz M, Barocke V, Braun D, Urtz N, Schulz C, von Bruhl M, Tirniceriu A, Gaertner F, Proia R, Graf T, Bolz S, Montanez E, Prinz M, Muller A, von Baumgarten L, Billich A, Sixt M, Fassler R, von Andrian U, Junt T, Massberg S A novel role of sphingosine 1-phosphate receptor S1pr1 in mouse thrombopoiesis. J Exp Med, 2012-11-12;209(12):2165-81. 2012-11-12 [PMID: 23148237] (Mouse)		Mouse
Mcl-1 and Bcl-xL regulate Bak/Bax-dependent apoptosis of the megakaryocytic lineage at multistages. Cell Death Differ., 2012-07-13;19(11):1856-69. 2012-07-13 [PMID: 22790873] (Mouse)		Mouse
Mcl-1 and Bcl-x(L) coordinately regulate megakaryocyte survival. Blood, 2012-02-28;119(24):5850-8. 2012-02-28 [PMID: 22374700] (Mouse)		Mouse
Salojin KV, Cabrera RM, Sun W A mouse model of hereditary folate malabsorption: deletion of the PCFT gene leads to systemic folate deficiency. Blood, 2011-02-23;117(18):4895-904. 2011-02-23 [PMID: 21346251] (Mouse)		Mouse
Heckl D, Wicke DC, Brugman MH, Meyer J, Schambach A, Busche G, Ballmaier M, Baum C, Modlich U Lentiviral gene transfer regenerates hematopoietic stem cells in a mouse model for Mpl-deficient aplastic anemia. Blood, 2011-02-02;117(14):3737-47. 2011-02-02 [PMID: 21289307] (Mouse)		Mouse
Peeters K, Loyen S, Van Kerckhoven S, Stoffels K, Hoylaerts MF, Van Geet C, Freson K Thrombopoietic effect of VPAC1 inhibition during megakaryopoiesis. Br. J. Haematol., 2010-08-05;151(1):54-61. 2010-08-05 [PMID: 20735399] (Mouse)		Mouse
Kruse EA, Loughran SJ, Baldwin TM, Josefsson EC, Ellis S, Watson DK, Nurden P, Metcalf D, Hilton DJ, Alexander WS, Kile BT Dual requirement for the ETS transcription factors Fli-1 and Erg in hematopoietic stem cells and the megakaryocyte lineage. Proc. Natl. Acad. Sci. U.S.A., 2009-07-31;106(33):13814-9. 2009-07-31 [PMID: 19666492] (Mouse)		Mouse
Eckly A, Strassel C, Freund M, Cazenave JP, Lanza F, Gachet C, Leon C Abnormal megakaryocyte morphology and proplatelet formation in mice with megakaryocyte-restricted MYH9 inactivation. Blood, 2008-11-04;113(14):3182-9. 2008-11-04 [PMID: 18984861] (Mouse)		Mouse
Lannutti BJ, Epp A, Roy J, Chen J, Josephson NC Incomplete restoration of Mpl expression in the mpl-/- mouse produces partial correction of the stem cell-repopulating defect and paradoxical thrombocytosis. Blood, 2008-09-16;113(8):1778-85. 2008-09-16 [PMID: 18796624] (Mouse)		Mouse
Zhang P, Nelson S, Bagby GJ, Siggins R, Shellito JE, Welsh DA The lineage-c-Kit+Sca-1+ cell response to Escherichia coli bacteremia in Balb/c mice. Stem Cells, 2008-05-15;26(7):1778-86. 2008-05-15 [PMID: 18483422] (Mouse)		Mouse
Xing S, Wanting TH, Zhao W, Ma J, Wang S, Xu X, Li Q, Fu X, Xu M, Zhao ZJ Transgenic expression of JAK2V617F causes myeloproliferative disorders in mice. Blood, 2008-03-11;111(10):5109-17. 2008-03-11 [PMID: 18334677] (Mouse)		Mouse
Tiedt R, Hao-Shen H, Sobas MA, Looser R, Dirnhofer S, Schwaller J, Skoda RC Ratio of mutant JAK2-V617F to wild-type Jak2 determines the MPD phenotypes in transgenic mice. Blood, 2007-12-26;111(8):3931-40. 2007-12-26 [PMID: 18160670] (Mouse)		Mouse
Kile BT, Panopoulos AD, Stirzaker RA, Hacking DF, Tahtamouni LH, Willson TA, Mielke LA, Henley KJ, Zhang JG, Wicks IP, Stevenson WS, Nurden P, Watowich SS, Justice MJ Mutations in the cofilin partner Aip1/Wdr1 cause autoinflammatory disease and macrothrombocytopenia. Blood, 2007-05-21;110(7):2371-80. 2007-05-21 [PMID: 17515402] (Mouse)		Mouse
Wu Y, Welte T, Michaud M, Madri JA PECAM-1: a multifaceted regulator of megakaryocytopoiesis. Blood, 2007-04-05;110(3):851-9. 2007-04-05 [PMID: 17412889] (Mouse)		Mouse
Kaluzhny Y, >”eêCY¦>”e, Yu G, Sun S, Toselli PA, Nieswandt B, Jackson CW, Ravid K BclxL overexpression in megakaryocytes leads to impaired platelet fragmentation. 2002-09-01;100(5):1670-8. 2002-09-01 [PMID: 12176887] (Mouse)		Mouse
Kaser A, Brandacher G, Steurer W, Kaser S, Offner FA, Zoller H, Theurl I, Widder W, Molnar C, Ludwiczek O, Atkins MB, Mier JW, Tilg H Interleukin-6 stimulates thrombopoiesis through thrombopoietin: role in inflammatory thrombocytosis. Blood, 2001-11-01;98(9):2720-5. 2001-11-01 [PMID: 11675343] (Mouse)		Mouse
Shih CC, Hu MC, Hu J A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells. Blood, 2000-03-15;95(6):1957-66. 2000-03-15 [PMID: 10706861] (Mouse)		Mouse
Show All 43 Publications. Collapse Publications.

Array MTP00

• Thrombopoietin/THPO Antibodies
• Thrombopoietin/THPO ELISA Kit
• Thrombopoietin/THPO Lysate
• Thrombopoietin/THPO Protein
• Thrombopoietin/THPO Proteins and Enzymes
• Thrombopoietin/THPO ELISAs