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MLH3 Antibody (CL14084)

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Staining of human breast cancer (ductal carcinoma) shows strong nuclear positivity in tumor cells.
Staining of human breast cancer (ductal carcinoma) shows strong nuclear positivity in tumor cells.
Orthogonal Strategies: Analysis in human endometrium and skeletal muscle tissues using NBP3-24596 antibody. Corresponding MLH3 RNA-seq data are presented for the same tissues.
Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.
Staining of human rectum shows moderate nuclear positivity in glandular cells.
Staining of human skeletal muscle shows no nuclear positivity in myocytes as expected.
Staining of human ovarian cancer (high grade serous ovarian carcinoma) shows strong nuclear positivity in tumor cells.
Staining in A-431 cell line with Anti-MLH3 monoclonal antibody, showing specific staining of nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Staining of human fallopian tube shows moderate nuclear positivity in glandular cells.
Staining of human endometrium shows strong nuclear positivity in glandular cells.
Western blot analysis in human cell line SCLC-21H.
Staining of human endometrial cancer shows strong nuclear positivity in tumor cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
CL14084
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

  

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MLH3 Antibody (CL14084) Summary

Immunogen
This antibody was designed to target the recombinant protein MLH3, with the precise immunogen sequence being withheld for proprietary reasons.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
MLH3
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments, For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for MLH3 Antibody (CL14084)

  • DNA mismatch repair protein Mlh3
  • HNPCC7
  • MGC138372
  • mutL (E. coli) homolog 3
  • mutL homolog 3 (E. coli)
  • MutL protein homolog 3

Background

MLH3 is a member of the MutL-homolog (MLH) family of DNA mismatch repair (MMR) genes. MLH genes are implicated in maintaining genomic integrity during DNA replication and after meiotic recombination. The protein encoded by this gene functions as a heterodimer with other family members. Somatic mutations in this gene frequently occur in tumors exhibiting microsatellite instability, and germline mutations have been linked to hereditary nonpolyposis colorectal cancer type 7 (HNPCC7). Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MLH3