![Western Blot: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - GST-tagged wild-type MIG2/Kindlin-2 or MIG2/Kindlin-2 (S159A) were incubated without IKKE (-), or with purified wild-type IKKE (WT) or mutant IKKE (K38A) in kinase buffer and resolved by SDS-PAGE, followed by immunoblotting analysis with the indicated antibodies. Image collected and cropped by CiteAb from the following publication (//www.ncbi.nlm.nih.gov/pmc/articles/PMC7019159/) licensed under a CC-BY license](http://images.novusbio.com/fullsize/MIG2-Kindlin-2-Antibody-OTI9E4-Western-Blot-NBP1-47745-img0023.jpg "Western Blot: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - GST-tagged wild-type MIG2/Kindlin-2 or MIG2/Kindlin-2 (S159A) were incubated without IKKE (-), or with purified wild-type IKKE (WT) or mutant IKKE (K38A) in kinase buffer and resolved by SDS-PAGE, followed by immunoblotting analysis with the indicated antibodies. Image collected and cropped by CiteAb from the following publication (//www.ncbi.nlm.nih.gov/pmc/articles/PMC7019159/) licensed under a CC-BY license")
| Reactivity | Hu, Mu, Rt, Ca, PmSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clone | OTI9E4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Full-length protein expressed in 293T cell transfected with human FERMT2 expression vector |
| Specificity | This antibody is specific for Homo sapiens fermitin family homolog 2 (Drosophila) (FERMT2), transcript variant 1. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | FERMT2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 77.9 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
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| Publication using NBP1-47745 | Applications | Species |
|---|---|---|
| Liu G, Bao Y et al. IKK epsilon phosphorylates kindlin-2 to induce invadopodia formation and promote colorectal cancer metastasis. Theranostics 2020-02-28 [PMID: 32104508] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of HepG2 cells using anti-Kindlin-2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MIG2-Kindlin-2-Antibody-OTI9E4-Immunocytochemistry-Immunofluorescence-NBP1-47745-img0012.jpg "Immunocytochemistry/Immunofluorescence: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of HepG2 cells using anti-Kindlin-2 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of paraffin-embedded Human pancreas tissue using anti-Kindlin-2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MIG2-Kindlin-2-Antibody-OTI9E4-Immunohistochemistry-Paraffin-NBP1-47745-img0019.jpg "Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of paraffin-embedded Human pancreas tissue using anti-Kindlin-2 mouse monoclonal antibody.")
![Western Blot: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Kindlin-2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Kindlin-2.](http://images.novusbio.com/fullsize/MIG2-Kindlin-2-Antibody-OTI9E4-Western-Blot-NBP1-47745-img0020.jpg "Western Blot: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Kindlin-2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Kindlin-2.")
![Western Blot: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Kindlin-2 monoclonal antibody.](http://images.novusbio.com/fullsize/MIG2-Kindlin-2-Antibody-OTI9E4-Western-Blot-NBP1-47745-img0021.jpg "Western Blot: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Kindlin-2 monoclonal antibody.")
![Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-Kindlin-2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MIG2-Kindlin-2-Antibody-OTI9E4-Immunohistochemistry-Paraffin-NBP1-47745-img0013.jpg "Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-Kindlin-2 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Kindlin-2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MIG2-Kindlin-2-Antibody-OTI9E4-Immunohistochemistry-Paraffin-NBP1-47745-img0014.jpg "Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Kindlin-2 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-Kindlin-2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MIG2-Kindlin-2-Antibody-OTI9E4-Immunohistochemistry-Paraffin-NBP1-47745-img0015.jpg "Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-Kindlin-2 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Kindlin-2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MIG2-Kindlin-2-Antibody-OTI9E4-Immunohistochemistry-Paraffin-NBP1-47745-img0016.jpg "Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Kindlin-2 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of paraffin-embedded Human colon tissue using anti-Kindlin-2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MIG2-Kindlin-2-Antibody-OTI9E4-Immunohistochemistry-Paraffin-NBP1-47745-img0017.jpg "Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of paraffin-embedded Human colon tissue using anti-Kindlin-2 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of paraffin-embedded Human Ovary tissue using anti-Kindlin-2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MIG2-Kindlin-2-Antibody-OTI9E4-Immunohistochemistry-Paraffin-NBP1-47745-img0018.jpg "Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - Staining of paraffin-embedded Human Ovary tissue using anti-Kindlin-2 mouse monoclonal antibody.")
![Immunoprecipitation: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - (Negative control: IP without adding anti-FERMT2 antibody.). For each experiment, 500ul of DDK tagged FERMT2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-FERMT2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/MIG2-Kindlin-2-Antibody-OTI9E4-Immunoprecipitation-NBP1-47745-img0022.jpg "Immunoprecipitation: MIG2/Kindlin-2 Antibody (OTI9E4) [NBP1-47745] - (Negative control: IP without adding anti-FERMT2 antibody.). For each experiment, 500ul of DDK tagged FERMT2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-FERMT2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.")
![Western Blot SOS2 Antibody [Unconjugated]](https://images.novusbio.com/images2/SOS2_AF6260_Western_Blot_9542.jpg)
