Recombinant Human MGP His Protein Summary
Description |
A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 20-96 of Human MGP Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MYESHESMES YELNPFINRR NANTFISPQQ RWRAKVQERI RERSKPVHEL NREACDDYRL CERYAMVYGY NAAYNRYF |
Source |
E. coli |
Protein/Peptide Type |
Recombinant Protein |
Gene |
MGP |
Purity |
>90%, by SDS-PAGE |
Applications/Dilutions
Dilutions |
|
Application Notes |
Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays. |
Theoretical MW |
11.8 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
20 mM Tris-HCl buffer (pH 8.0), 10% glycerol |
Preservative |
No Preservative |
Concentration |
1 mg/ml |
Purity |
>90%, by SDS-PAGE |
Alternate Names for Recombinant Human MGP His Protein
Background
MGP, also known as Matrix Gla protein, is a protein involved in regulating calcification in the extracellular matrix, in particular in cartilage and arteries. MGP is a vitamin K-dependent protein, and contains five to six residues of Gla, a Ca2+ binding amino acid requiring vitamin K-dependent gamma carboxylase for its formation. It is found in human bone extracts, likely formed by COOH-terminal processing by carboxypeptidase B-like enzymatic activity. Recombinant human MGP protein, fused to His-tag at N-terminus, was expressed in E.coli and denatured using detergent during a conventional chromatography purification process.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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