![Immunocytochemistry/Immunofluorescence: MED21 Antibody (PCRP-MED21-4B5) [NBP3-13814] - Immunofluorescence Analysis of PFA-fixed U87 cells stained using MED21 Antibody (PCRP-MED21-4B5) followed by goat anti-mouse IgG-CF488. Membrane stained with phalloidin.](http://images.novusbio.com/fullsize/MED21-Antibody-PCRP-MED21-4B5-Immunocytochemistry-Immunofluorescence-NBP3-13814-img0004.jpg "Immunocytochemistry/Immunofluorescence: MED21 Antibody (PCRP-MED21-4B5) [NBP3-13814] - Immunofluorescence Analysis of PFA-fixed U87 cells stained using MED21 Antibody (PCRP-MED21-4B5) followed by goat anti-mouse IgG-CF488. Membrane stained with phalloidin.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IP, MA |
| Clone | PCRP-MED21-4B5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14069) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Immunogen | Recombinant full-length human MED21 protein (Uniprot: Q13503) |
| Localization | Nucleus, Nucleoplasm, Cytoplasm |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | MED21 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | ELISA: For coating, order antibody without BSA Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate) |
| Theoretical MW | 15.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for MED21 Antibody (NBP3-13814)Find related products by research area.
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![Flow Cytometry: MED21 Antibody (PCRP-MED21-4B5) [NBP3-13814] - Flow cytometric analysis of PFA-fixed U87 cells. MED21 antibody (PCRP-MED21-4B5) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).](http://images.novusbio.com/fullsize/MED21-Antibody-PCRP-MED21-4B5-Flow-Cytometry-NBP3-13814-img0002.jpg "Flow Cytometry: MED21 Antibody (PCRP-MED21-4B5) [NBP3-13814] - Flow cytometric analysis of PFA-fixed U87 cells. MED21 antibody (PCRP-MED21-4B5) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).")
![Immunocytochemistry/Immunofluorescence: MED21 Antibody (PCRP-MED21-4B5) [NBP3-13814] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using MED21 Antibody (PCRP-MED21-4B5) followed by goat anti-mouse IgG-CF488. Membrane stained with phalloidin.](http://images.novusbio.com/fullsize/MED21-Antibody-PCRP-MED21-4B5-Immunocytochemistry-Immunofluorescence-NBP3-13814-img0003.jpg "Immunocytochemistry/Immunofluorescence: MED21 Antibody (PCRP-MED21-4B5) [NBP3-13814] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using MED21 Antibody (PCRP-MED21-4B5) followed by goat anti-mouse IgG-CF488. Membrane stained with phalloidin.")
![Flow Cytometry: MED21 Antibody (PCRP-MED21-4B5) [NBP3-13814] - Flow cytometric analysis of PFA-fixed HeLa cells. MED21 antibody (PCRP-MED21-4B5) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).](http://images.novusbio.com/fullsize/MED21-Antibody-PCRP-MED21-4B5-Flow-Cytometry-NBP3-13814-img0001.jpg "Flow Cytometry: MED21 Antibody (PCRP-MED21-4B5) [NBP3-13814] - Flow cytometric analysis of PFA-fixed HeLa cells. MED21 antibody (PCRP-MED21-4B5) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).")
![Protein Array: MED21 Antibody (PCRP-MED21-4B5) [NBP3-13814] - Analysis of Protein Array containing more than 19,000 full-length human proteins using MED21 Antibody (PCRP-MED21-4B5).](http://images.novusbio.com/fullsize/MED21-Antibody-PCRP-MED21-4B5-Protein-Array-NBP3-13814-img0006.jpg "Protein Array: MED21 Antibody (PCRP-MED21-4B5) [NBP3-13814] - Analysis of Protein Array containing more than 19,000 full-length human proteins using MED21 Antibody (PCRP-MED21-4B5).")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")