![Western Blot: MDG1 Antibody [NBP1-78785] - Staining of Human Liver (A), Kidney (B) and -Mouse Liver (C) lysate (35 ug protein in RIPA buffer) with antibody at 1 ug/mL. Detected by chemiluminescence. Approx 26-27 kDa band observed in Human Liver and Kidney lysates and approx. 28 kDa in Mouse Liver lysates (calculated MW of 25.5 kDa according to Human NP_036460.1 and 25.6 kDa according to Mouse NP_038788.2).](http://images.novusbio.com/fullsize/MDG1-Antibody-Western-Blot-NBP1-78785-img0001.jpg "Western Blot: MDG1 Antibody [NBP1-78785] - Staining of Human Liver (A), Kidney (B) and -Mouse Liver (C) lysate (35 ug protein in RIPA buffer) with antibody at 1 ug/mL. Detected by chemiluminescence. Approx 26-27 kDa band observed in Human Liver and Kidney lysates and approx. 28 kDa in Mouse Liver lysates (calculated MW of 25.5 kDa according to Human NP_036460.1 and 25.6 kDa according to Mouse NP_038788.2).")
| Reactivity | Hu, Mu, Rt, Po, Bv, CaSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | This MDG1 Antibody was developed against a peptide with sequence C-PDAEAKFREIAEAYE corresponding to internal region according to NP_036460.1. |
| Predicted Species | Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | DNAJB9 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
![Immunohistochemistry-Paraffin IRE1 alpha [p Ser724] Antibody - BSA Free](https://images.novusbio.com/images/IRE1-alpha-[p-Ser724]-Antibody-Immunohistochemistry-Paraffin-NB100-2323-img0025.jpg)
![Western Blot IRE1 alpha [p Ser724] Antibody - BSA Free](https://images.novusbio.com/images/IRE1-alpha-[p-Ser724]-Antibody-Western-Blot-NB100-2323-img0027.jpg)
![Western Blot IRE1 alpha [p Ser724] Antibody - BSA Free](https://images.novusbio.com/images/IRE1-alpha-[p-Ser724]-Antibody-Western-Blot-NB100-2323-img0029.jpg)
| Publication using NBP1-78785 | Applications | Species |
|---|---|---|
| Ushioda R, Hoseki J, Nagata K et al. Glycosylation-independent ERAD pathway serves as a backup system under ER stress. Mol Biol Cell 2013-08-21 [PMID: 23966469] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | DNAJB9 |
| Entrez |
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| Uniprot |
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![Immunocytochemistry/Immunofluorescence: MDG1 Antibody [NBP1-78785] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing endoplasmic reticulum/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/MDG1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78785-img0003.jpg "Immunocytochemistry/Immunofluorescence: MDG1 Antibody [NBP1-78785] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing endoplasmic reticulum/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Flow Cytometry: MDG1 Antibody [NBP1-78785] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/MDG1-Antibody-Flow-Cytometry-NBP1-78785-img0004.jpg "Flow Cytometry: MDG1 Antibody [NBP1-78785] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.")
![Immunocytochemistry/Immunofluorescence: MDG1 Antibody [NBP1-78785] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/MDG1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78785-img0002.jpg "Immunocytochemistry/Immunofluorescence: MDG1 Antibody [NBP1-78785] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Immunohistochemistry BMP-3b/GDF-10 Antibody [Unconjugated]](https://images.novusbio.com/images/antibody/af1543_human-bmp-3b-gdf-10-affinity-purified-polyclonal-ab-immunocytochemistry-133202414504.jpg)
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