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MBD1 Antibody (10B10)

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Immunocytochemistry/ Immunofluorescence: MBD1 Antibody (10B10) [NBP3-26147] - Staining of MCF-7 cell with MBD1 Antibody (10B10) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, ICC/IF
Clone
10B10
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

MBD1 Antibody (10B10) Summary

Additional Information
Recombinant monoclonal antibody expressed in HEK293F cells
Immunogen
A synthesized peptide derived from Human MBD1 [UniProt Q9UIS9]
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
MBD1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200

Packaging, Storage & Formulations

Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.4, 150mM NaCl, and 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for MBD1 Antibody (10B10)

  • CXXC-type zinc finger protein 3
  • methyl-CpG binding domain protein 1 isoform PCM1
  • methyl-CpG binding domain protein 1
  • methyl-CpG-binding domain protein 1
  • Methyl-CpG-binding protein MBD1
  • PCM1CXXC3RFT
  • Protein containing methyl-CpG-binding domain 1
  • the regulator of fibroblast growth factor 2 (FGF-2) transcription

Background

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters; in addition, variants with three CXXC domains also repress unmethylated promoter activity. MBD1 and MBD2 map very close to each other on chromosome 18q21.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Array NBP3-26147

Research Areas for MBD1 Antibody (NBP3-26147)

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Bioinformatics

Gene Symbol MBD1