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MARCKS Antibody

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Western Blot: MARCKS Antibody [NBP3-05540] - Western blot analysis of different cell lines lysates using MARCKS, at a dilution of 1:1,000 in green: [1] protein standard (red), [2] HEK293, [3] HeLa, [4] SH-SY5Y, and [5] ...read more
Immunocytochemistry/ Immunofluorescence: MARCKS Antibody [NBP3-05540] - Immunofluorescent analysis of HeLa cells stained with MARCKS Antibody at a dilution of 1:5,000 in red, and costained with mouse mAb to beta-tubulin ...read more

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated

Order Details

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MARCKS Antibody Summary

Immunogen
Full length recombinant human MARCKS expressed in and purified from E. coli.
Isotype
IgY
Clonality
Polyclonal
Host
Chicken
Gene
MARCKS
Purity
IgY purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:500-1:1000
  • Immunohistochemistry 1:500-1:1000
  • Western Blot 1:5000-1:10000
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
Supplied as concentrated total IgY preparation from egg yolk. Exact concentration of target specific IgY is not quantifiable as the preparation contains both immune IgY specific for the target and also irrelevant, non-immune IgY.
Preservative
0.035% Sodium Azide
Purity
IgY purified

Notes

Chicken products cannot be exported to Canada.

Alternate Names for MARCKS Antibody

  • 80K-L protein
  • 80K-L
  • FLJ14368
  • MACS
  • MACSmyristoylated alanine-rich C-kinase substrate
  • MARCKS
  • MRACKS
  • myristoylated alanine-rich protein kinase C substrate (MARCKS, 80K-L)
  • myristoylated alanine-rich protein kinase C substrate
  • phosphomyristin
  • PKCSLFLJ90045
  • PRKCSL
  • Protein kinase C substrate, 80 kDa protein, light chain

Background

Myristolyated alanine rich C-kinase substrate, hence MARCKS, was originally discovered as a major substrate for protein kinase C in the brain and other tissues, and was originally isolated from human epithelial cells. MARCKS is a major protein of brain, and is concentrated in synapses of neurons. It appears to function in synaptic vesicle cycling and has been shown to bind both actin and calmodulin in vitro. MARCKS belongs to a family of proteins with similar actin and calmodulin binding properties. Deletion of the MARCKS gene in mice results in embryonic brain defects and death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MARCKS