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MAP3K6 Antibody

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Immunocytochemistry/Immunofluorescence: MAP3K6 Antibody [NBP2-14219] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleus & cytosol.
Immunocytochemistry/ Immunofluorescence: MAP3K6 Antibody [NBP2-14219] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleus & cytosol.
Immunohistochemistry-Paraffin: MAP3K6 Antibody [NBP2-14219] - Staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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MAP3K6 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: EEPASPEESSGLSLLHQESKRRAMLAAVLEQELPALAENLHQEQKQEQGARLGRNHVEELLRCLGAHIHTPNRR
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAP3K6
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
MAP3K6 Protein (NBP2-14219PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%), Rat (84%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MAP3K6 Antibody

  • apoptosis signal regulating kinase 2
  • ASK2MGC20114
  • EC 2.7.11
  • EC 2.7.11.25
  • MAPKKK6MEKK6Apoptosis signal-regulating kinase 2
  • MGC125653
  • mitogen-activated protein kinase kinase kinase 6

Background

MAP3K6 encodes a member of the serine/threonine protein kinase family. The encoded kinase was identified by its interaction with MAP3K5/ASK, a protein kinase and an activator of c-Jun kinase (MAPK7/JNK) and MAPK14/p38 kinase. This kinase was found to weakly activate MAPK7, but not MAPK1/ERK or MAPK14. An alternatively spliced transcript variant has been found for this gene, but its biological validity has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MAP3K6