![Immunoprecipitation: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (PSH03-97) [NBP3-32495] - Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B was immunoprecipitated in 0.2mg A549 cell lysate with NBP3-32495 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32495 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: A549 cell lysate (input) Lane 2: IP in A549 cell lysate Lane 3: Rabbit IgG in A549 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds](http://images.novusbio.com/fullsize/nbp3-32495_rabbit-lysine-k-specific-demethylase-5b-kdm5b-jarid1b-mab-26820241582615.jpg "Immunoprecipitation: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (PSH03-97) [NBP3-32495] - Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B was immunoprecipitated in 0.2mg A549 cell lysate with NBP3-32495 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32495 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: A549 cell lysate (input) Lane 2: IP in A549 cell lysate Lane 3: Rabbit IgG in A549 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IP |
| Clone | PSH03-97 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Synthetic peptide within Human Lysine (K)-specific Demethylase 5B/Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B aa 960-1,009 / 1,544. (Uniprot: Q9UGL1) |
| Localization | Nucleus. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | KDM5B |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 176 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH7.4), 0.1% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (NBP3-32495)Find related products by research area.
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| Gene Symbol | KDM5B |
![Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (PSH03-97) [NBP3-32495] - Western blot analysis of Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B on different lysates with Rabbit anti-Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B antibody (NBP3-32495) at 1/2,000 dilution. Lane 1: BT-20 cell lysate Lane 2: MDA-MB-468 cell lysate Lane 3: AGS cell lysate Lane 4: A549 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 176 kDa Observed band size: 145 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32495) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32495_rabbit-lysine-k-specific-demethylase-5b-kdm5b-jarid1b-mab-278202416331293.jpg "Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (PSH03-97) [NBP3-32495] - Western blot analysis of Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B on different lysates with Rabbit anti-Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B antibody (NBP3-32495) at 1/2,000 dilution. Lane 1: BT-20 cell lysate Lane 2: MDA-MB-468 cell lysate Lane 3: AGS cell lysate Lane 4: A549 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 176 kDa Observed band size: 145 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32495) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/200,000 dilution was used for 1 hour at room temperature.")
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