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LRP-4 Antibody [Unconjugated]

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Western blot shows lysates of human brain (cortex) tissue and rat cortical embryonic neuron/glial cells. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Rat LRP-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5948) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LRP-4 at approximately 220 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Western blot shows lysates of human brain (cortex) tissue and rat cortical embryonic neuron/glial cells. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Rat LRP-4 Antigen Affinity-purified Polyclonal Antibody ...read more
LRP-4 was detected in immersion fixed paraffin-embedded sections of human brainstem using Sheep Anti-Human/Rat LRP-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5948) at 3 µg/mL overnight at 4 °C. ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF5948-SP
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LRP-4 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human LRP-4
Gly18-Ser344
Accession # O75096
Specificity
Detects human and rat LRP-4 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) LRP-1, rhLRP-1B, rhLRP-5, rhLRP-6, and rhLDL R is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
LRP4
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LRP-4 Antibody [Unconjugated]

  • CLSS
  • KIAA0816
  • low density lipoprotein receptor-related protein 4
  • low-density lipoprotein receptor-related protein 4
  • LRP10
  • LRP4
  • LRP-4
  • MEGF7LRP10
  • Multiple epidermal growth factor-like domains 7

Background

LRP‑4 (Low density lipoprotein-related protein #4; also MEGF7 and LRP13) is a 220-270 kDa glycoprotein, member of the LDLR family of proteins. It is expressed on neurons, oocytes, spermatogonia and skeletal muscle cells, and binds multiple ligands, including WISE, apoE, MuSK and neuronal Agrin. It serves to negatively regulate Wnt signaling during development, and to cluster AChRs at neuromuscular junctions. Mature human LRP‑4 is an 1885 amino acid (aa) type I transmembrane glycoprotein. It contains a 1705 aa extracellular domain (ECD) (aa 21-1725) plus a 159 aa cytoplasmic region (aa 1747-1905).  In the ECD, there are eight LDLR class A repeats (aa 26-350), two EGF-like repeats (aa 354-434) and 20 LDLR class B repeats that contain an intervening EGF-like domain (aa 480-1610). Over aa 18-344, human LRP‑4 shares 88% aa identity with mouse LRP‑4.        

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol LRP4
Uniprot