| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, IHC |
| Clone | 741704 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | E. coli-derived recombinant human LRP-4 Gly18-Ser344 Accession # O75096 |
| Specificity | Detects human LRP-4 in direct ELISAs and Western blots.
In direct ELISAs and Western blots, no
cross-reactivity with recombinant human (rh) LRP-5 or rhLRP-6 is observed. |
| Source | N/A |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | LRP4 |
| Purity Statement | Protein A or G purified from hybridoma culture supernatant |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Publications |
|
| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Reconstitution Instructions | Sterile PBS to a final concentration of 0.5 mg/mL. |
LRP‑4 (Low density lipoprotein-related protein #4; also MEGF7 and LRP13) is a 220-270 kDa glycoprotein, member of the LDLR family of proteins. It is expressed on neurons, oocytes, spermatogonia and skeletal muscle cells and binds multiple ligands including WISE, apoE, MuSK and neuronal Agrin. It serves to negatively regulate Wnt signaling during development and to cluster AChRs at neuromuscular junctions. Mature human LRP‑4 is an 1885 amino acid (aa) type I transmembrane glycoprotein. It contains a 1705 aa extracellular domain (ECD) (aa 21-1725) plus a 159 aa cytoplasmic region (aa 1747-1905). In the ECD, there are eight LDLR class A repeats (aa 26-350), two EGF-like repeats (aa 354-434) and 20 LDLR class B repeats that contain an intervening EGF-like domain (aa 480-1610). Over aa 18-344, human LRP‑4 shares 97% aa identity with both mouse and rat LRP‑4. MAB5948 detects the correct rat band in Western Blot. Immunohistochemistry was not tested in rat samples.
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunohistochemistry LDLR Antibody [Unconjugated]](https://images.novusbio.com/images2/LDL_R_AF2255_Immunohistochemistry_7149.jpg)
![Western Blot LDLR Antibody [Unconjugated]](https://images.novusbio.com/images2/LDLR_AF2255_Western_Blot_23731.jpg)
![Western Blot LDLR Antibody [Unconjugated]](https://images.novusbio.com/images/af2255_mouse-ldl-r-affinity-purified-polyclonal-ab-4120241049445.jpg)
![Agrin [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Agrin [Unconjugated]")
![Immunohistochemistry SOST/Sclerostin Antibody [Unconjugated]](https://images.novusbio.com/images2/SOST_AF1589_Immunohistochemistry_7131.jpg)
![Immunohistochemistry SOST/Sclerostin Antibody [Unconjugated]](https://images.novusbio.com/images2/SOST_AF1589_Immunohistochemistry_7132.jpg)
![Western Blot SOST/Sclerostin Antibody [Unconjugated]](https://images.novusbio.com/images2/SOST_AF1589_Western_Blot_19262.jpg)
![Flow Cytometry LRP-6 Antibody (255302) [Unconjugated]](https://images.novusbio.com/images2/LRP-6_MAB1505_Flow_Cytometry_11214.jpg)
![Immunocytochemistry LRP-6 Antibody (255302) [Unconjugated]](https://images.novusbio.com/images2/LRP-6_MAB1505_Immunocytochemistry_11355.jpg)
