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LIN7B Antibody (OTI1C9) - Azide and BSA Free

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Western Blot: LIN7B Antibody (OTI1C9) - Azide and BSA Free [NBP2-72131] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIN7B (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunocytochemistry/ Immunofluorescence: LIN7B Antibody (OTI1C9) - Azide and BSA Free [NBP2-72131] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY LIN7B.
Immunohistochemistry: LIN7B Antibody (OTI1C9) - Azide and BSA Free [NBP2-72131] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-LIN7B mouse monoclonal antibody.
Flow Cytometry: LIN7B Antibody (OTI1C9) - Azide and BSA Free [NBP2-72131] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-LIN7B ...read more
Immunohistochemistry: LIN7B Antibody (OTI1C9) - Azide and BSA Free [NBP2-72131] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-LIN7B mouse monoclonal antibody.
Immunohistochemistry: LIN7B Antibody (OTI1C9) - Azide and BSA Free [NBP2-72131] - Staining of paraffin-embedded Human lymph node tissue using anti-LIN7B mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
OTI1C9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

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LIN7B Antibody (OTI1C9) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human LIN7B (NP_071448) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LIN7B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
22.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for LIN7B Antibody (OTI1C9) - Azide and BSA Free

  • hLin7B
  • lin-7 homolog B (C. elegans)
  • LIN-7B
  • MALS-2protein lin-7 homolog B
  • MALS2veli-2
  • Mammalian lin-seven protein 2
  • Veli-2
  • VELI2hVeli2
  • Vertebrate lin-7 homolog 2

Background

LIN7B plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at theplasma membrane of polarized cells. Forms membrane-associated multiprotein complexes that may regulate delivery andrecycling of proteins to the correc

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LIN7B Antibody (NBP2-72131) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol LIN7B