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Lgr6 Antibody (OTI2A3) - Azide and BSA Free

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Western Blot: Lgr6 Antibody (OTI2A3) - Azide and BSA Free [NBP2-72119] - Analysis of HEK293T cells lysate (5 ug, left lane) and Analysis of HEK293T cells lysate expressing human recombinant protein fragment (5 ug, right ...read more
Immunocytochemistry/ Immunofluorescence: Lgr6 Antibody (OTI2A3) - Azide and BSA Free [NBP2-72119] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY LGR6.
Western Blot: Lgr6 Antibody (OTI2A3) - Azide and BSA Free [NBP2-72119] - Analysis of extracts (10ug) from 8 Human tissue (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clone
OTI2A3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

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Lgr6 Antibody (OTI2A3) - Azide and BSA Free Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 25-250 of human LGR6(NP_001017403) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LGR6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000
Theoretical MW
104.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Lgr6 Antibody (OTI2A3) - Azide and BSA Free

  • FLJ14471
  • gonadotropin receptor
  • GPCR
  • leucine-rich repeat containing G protein-coupled receptor 6
  • leucine-rich repeat-containing G protein-coupled receptor 6
  • leucine-rich repeat-containing G-protein coupled receptor 6
  • Lgr6
  • VTS20631

Background

LGR6 has been reported to be expressed in rat adrenal, brain, colon, heart, kidney, ovary, oviduct, small intestine, spleen, testis, and uterus Hsu et al., 2000). ESTs have been isolated from human B-cell/lung/testis, blood, brain, colon, eye, germ cell, head/neck, heart, heart/melanocyte/uterus, lung, ovary, stomach, testis, and uterus libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol LGR6