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Lgi1 Antibody

Images

 
Western Blot: Lgi1 Antibody [NBP1-33087] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LGI1 antibody [N2C2], Internal diluted at 1:1000. The HRP-conjugated ...read more
Immunocytochemistry/ Immunofluorescence: Lgi1 Antibody [NBP1-33087] - LGI1 antibody [N2C2], Internal detects LGI1 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary hippocampal neurons were fixed in 4% ...read more
Western Blot: Lgi1 Antibody [NBP1-33087] - Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE, antibody diluted at 1:5000.
Western Blot: Lgi1 Antibody [NBP1-33087] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with LGI1 antibody [N2C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit ...read more

Product Details

Summary
Reactivity Hu, Rt, Mu, Bv, Pm, RMSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Lgi1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Lgi1. The exact sequence is proprietary.
Localization
Secreted
Predicted Species
Mouse (95%), Rhesus Macaque (100%), Bovine (98%), Chimpanzee (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LGI1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:10000
Theoretical MW
64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (86%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for Lgi1 Antibody

  • ADLTE
  • ADPAEF
  • ADPEAF
  • BB130740
  • epilepsy, partial
  • Epitempin
  • Epitempin-1
  • EPT
  • EPTleucine-rich glioma-inactivated protein 1
  • ETL1
  • ETL1epitempin-1
  • IB1099
  • leucine-rich, glioma inactivated 1
  • LGI1

Background

This gene is rearranged as a result of translocations in glioblastoma cell lines. The protein contains a hydrophobic segment representing a putative transmembrane domain with the amino terminus located outside the cell. It also contains leucine-rich repeats with conserved cysteine-rich flanking sequences. This gene is predominantly expressed in neural tissues and its expression is reduced in low grade brain tumors and significantly reduced or absent in malignant gliomas. Mutations in this gene result in autosomal dominant lateral temporal epilepsy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Lgi1 Antibody (NBP1-33087) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol LGI1