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LAT Antibody

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Western Blot: LAT Antibody [NB100-1441] - (0.01ug/ml) staining of peripheral blood mononucleocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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LAT Antibody Summary

Immunogen
Peptide with sequence C-GAPDYENLQELN corresponding to C-Terminus according to NP_055202.1, NP_001014987.1, NP_001014989.1, NP_001014988.1.
Specificity
This antibody is expected to recognize isoform a (NP_055202.1), isoform b (NP_001014987.1 and NP_001014989.1) and isoform c (NP_001014988.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LAT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:2000
  • Western Blot 0.01 - 0.03 ug/ml
Application Notes
WB: Approx. 37 kDa band observed in human lymph node and peripheral Brood mononucleocytes lysates and in mouse and rat thymus lysates (calculated MW of 27.9 kDa band according to NP_055202.1). The observed molecular weight corresponds to earlier findings with different antibodies from other sources.
Publications
Read Publication using NB100-1441.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for LAT Antibody

  • 36 kDa phospho-tyrosine adapter protein
  • LAT
  • LAT1,36 kDa phospho-tyrosine adaptor protein
  • linker for activation of T cells
  • linker for activation of T-cells family member 1
  • p36-38
  • pp36

Background

Linker for activation of T cells (LAT) is an integral membrane protein (36 to 38 kD), which plays an important role in linking engagement of the TCR to the biochemical events of T cell activation. LAT antibody stains thymus and peripheral lymphoid tissues such as T cell areas in lymph nodes and spleen. LAT is expressed in T lymphocytes in interstitial spaces, platelets and megakaryocytes. LAT is not expressed in B cells, macrophages, plasma cells, monocytes, epithelial histiocytes and dendritic cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LAT Antibody (NB100-1441)(1)


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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional LAT Products

Research Areas for LAT Antibody (NB100-1441)

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Blogs on LAT.

Cluster of Differentiation 3 (CD3) (OKT3 clone) as a Marker of Immune Response Efficiency
Our immune system is a powerful defense mechanism against infection, however different variables can cause our immune response to work for or against us.  CD3 (cluster of differentiation 3) is one component of our immune signal response that is co...  Read full blog post.

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Bioinformatics

Gene Symbol LAT
Entrez
Uniprot