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L1CAM Antibody (L1-14.10) [Janelia Fluor® 585]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP
Clone
L1-14.10
Clonality
Monoclonal
Host
Rabbit
Conjugate
Janelia Fluor 585

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-12057AF350
Alexa Fluor 405
NBP3-12057AF405
Alexa Fluor 488
NBP3-12057AF488
Alexa Fluor 532
NBP3-12057AF532
Alexa Fluor 594
NBP3-12057AF594
Alexa Fluor 647
NBP3-12057AF647
Alexa Fluor 700
NBP3-12057AF700
Alexa Fluor 750
NBP3-12057AF750
Allophycocyanin
NBP3-12057APC
Biotin
NBP3-12057B
CoraFluor 1
NBP3-12057CL1
DyLight 350
NBP3-12057UV
DyLight 405
NBP3-12057V
DyLight 488
NBP3-12057G
DyLight 550
NBP3-12057R
DyLight 594
NBP3-12057DL594
DyLight 650
NBP3-12057C
DyLight 680
NBP3-12057FR
DyLight 755
NBP3-12057IR
FITC
NBP3-12057F
HRP
NBP3-12057H
Janelia Fluor 525
NBP3-12057JF525
Janelia Fluor 549
NBP3-12057JF549
Janelia Fluor 635
NBP3-12057JF635
Janelia Fluor 646
NBP3-12057JF646
Janelia Fluor 669
NBP3-12057JF669
PE
NBP3-12057PE
PerCP
NBP3-12057PCP
Unconjugated
NBP3-12057-0.2mg
mFluor Violet 450 SE
NBP3-12057MFV450
mFluor Violet 500 SE
NBP3-12057MFV500
mFluor Violet 610 SE
NBP3-12057MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP3-12057-0.2mg
Azide and BSA Free
NBP3-12057-0.2mg
Azide and BSA Free
NBP3-12057-0.025mg
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L1CAM Antibody (L1-14.10) [Janelia Fluor® 585] Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
This antibody was raised by immunising mice with recombinant L1-Fc fusion protein, consisting of the ectodomain of human L1.
Specificity
This antibody is specific for the ectodomain (amino acids M1 to E1123) of human L1, and recognises an epitope in the third Ig domain. L1 is over-expressed in a range of tumours, including neuroblastomas, renal carcinomas, ovarian and endometrial carcinomas, and melanomas. This antibody does not cross-react with human CHL1.
Isotype
IgG Kappa
Clonality
Monoclonal
Host
Rabbit
Gene
L1CAM
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for L1CAM Antibody (L1-14.10) [Janelia Fluor® 585]

  • antigen identified by monoclonal R1
  • CAML1
  • CAML1N-CAML1
  • CD171 antigen
  • CD171
  • HSAS
  • HSAS1
  • L1 cell adhesion molecule
  • L1CAM
  • MASA
  • MIC5
  • N-CAM-L1
  • NCAM-L1
  • neural cell adhesion molecule L1
  • S10
  • SPG1

Background

The L1CAM gene, which is located in Xq28, is involved in three distinct conditions: 1) HSAS (hydrocephalus-stenosis of the aqueduct of Sylvius); 2) MASA (mental retardation, aphasia, shuffling gait, adductus thumbs); and 3) SPG1 (spastic paraplegia). The L1, neural cell adhesion molecule (L1CAM) also plays an important role in axon growth, fasciculation, neural migration and in mediating neuronal differentiation. Expression of L1 protein is restricted to tissues arising from neuroectoderm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol L1CAM